12111-1-AP

antibody from Invitrogen Antibodies

Targeting: DPF2 BAF45d, REQ, ubi-d4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12111-1-AP

Provider

Invitrogen Antibodies

Product name

DPF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAAVVENVV KLLGEQYYKD AMEQCHNYNA RLCAERSVRL PFLDSQTGVA QSNCYIWMEK RHRGPGLASG QLYSYPARRW RKKRRAHPPE DPRLSFPSIK PDTDQTLKKE GLISQDGSSL EALLRTDPLE KRGAPDPRVD DDSLGEFPVT NSRARKRILE PDDFLDDLDD EDYEEDTPKR RGKGKSKGKG VGSARKKLDA SILEDRDKPY ACDICGKRYK NRPGLSYHYA HSHLAEEEGE DKEDSQPPTP VSQRSEEQKS KKGPDGLALP NNYCDFCLGD SKINKKTGQP EELVSCSDCG RSGHPSCLQF TPVMMAAVKT YRWQCIECKC CNICGTSEND DQLLFCDDCD RGYHMYCLTP SMSEPPEGSW SCHLCLDLLK EKASIYQNQN SS (1-391 aa encoded by BC014889)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.16 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12111-1-AP (DPF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 12111-1-AP (DPF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various cells were subjected to SDS PAGE followed by western blot with 12111-1-AP (DPF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 12111-1-AP (DPF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using DPF2 antibody 12111-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using DPF2 antibody 12111-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pituitary adenoma tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pituitary adenoma tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 12111-1-AP (DPF2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 12111-1-AP (DPF2 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DPF2 (IP:12111-1-AP, 4ug; Detection:12111-1-AP 1:500) with Jurkat cells lysate 1200ug.