PAB21100

antibody from Abnova Corporation

Targeting: CDCA7 FLJ14736, JPO1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21100

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21100, RRID:AB_10985305

Product name

CDCA7 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CDCA7.

Antigen sequence

PKFRSDISEELANVFYEDSDNESFCGFSESEVQDV
LDHCGFLQKPRPDVTNELAGIFHADSDDESFCGFS
ESEIQDGM


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with CDCA7 polyclonal antibody (Cat # PAB21100) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with CDCA7 polyclonal antibody (Cat # PAB21100) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with CDCA7 polyclonal antibody (Cat # PAB21100) shows strong cytoplasmic positivity in granular pattern in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)