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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
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- Product number
- PA5-52165 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDCA7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PKFRSDISEE LANVFYEDSD NESFCGFSES EVQDVLDHCG FLQKPRPDVT NELAGIFHAD SDDESFCGFS ESEIQDGM
- Concentration
- 0.1 mg/mL
Submitted references High expression of CDCA7 predicts tumor progression and poor prognosis in human colorectal cancer.
Li S, Huang J, Qin M, Zhang J, Liao C
Molecular medicine reports 2020 Jul;22(1):57-66
Molecular medicine reports 2020 Jul;22(1):57-66
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CDCA7 in human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a CDCA7 Polyclonal Antibody (Product # PA5-52165).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CDCA7 in human cell line U-251 MG using a CDCA7 Polyclonal Antibody (Product # PA5-52165) shows localization to nucleoplasm and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Levels of CDCA7 in CRC tissues. (A) Immunohistochemistry streptavidin peroxidase staining was conducted to determine the levels of CDCA7 in CRC tissues and paired adjacent normal colorectal tissues (magnification, x400). (B) Relative mRNA expression levels of CDCA7 in 15 pairs of CRC tissues and adjacent normal colorectal tissues were assessed by reverse transcription-quantitative PCR and (C) western blotting. (D) Relative protein levels of CDCA7 in 15 CRC tissues and their paired adjacent normal colorectal tissues. GAPDH was used as the internal control. Data were examined by t-test and are presented as the mean +- SD. CDCA7, cell division cycle associated protein 7; CRC, colorectal cancer.
