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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-52117 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-52117, RRID:AB_11020260
- Product name
- Goat Polyclonal IDH2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Goat
- Antigen sequence
CIHGLSNVKLNE- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Acquired mutations in the genes encoding IDH1 and IDH2 both are recurrent aberrations in acute myeloid leukemia: prevalence and prognostic value.
Abbas S, Lugthart S, Kavelaars FG, Schelen A, Koenders JE, Zeilemaker A, van Putten WJ, Rijneveld AW, Löwenberg B, Valk PJ
Blood 2010 Sep 23;116(12):2122-6
Blood 2010 Sep 23;116(12):2122-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IDH2 Antibody [NBP1-52117] - Analysis of IDH2 in Human Heart lysate (35ug protein in RIPA buffer) using NBP1-52117 at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IDH2 Antibody [NBP1-52117] - 0.1ug/ml staining of Mouse (A), Rat (B) and Pig (C) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-52117] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
