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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00007392-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007392-M01, RRID:AB_549056
- Product name
- Mouse Monoclonal USF2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. USF2 - upstream transcription factor 2, c-fos interacting
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Transcriptome analysis reveals a dynamic and differential transcriptional response to sulforaphane in normal and prostate cancer cells and suggests a role for Sp1 in chemoprevention.
Beaver LM, Buchanan A, Sokolowski EI, Riscoe AN, Wong CP, Chang JH, Löhr CV, Williams DE, Dashwood RH, Ho E
Molecular nutrition & food research 2014 Oct;58(10):2001-13
Molecular nutrition & food research 2014 Oct;58(10):2001-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USF2 Antibody (5E9) [H00007392-M01] - Analysis of USF2 expression in transfected 293T cell line by USF2 monoclonal antibody (M01), clone 5E9.Lane 1: USF2 transfected lysate(37 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USF2 Antibody (5E9) [H00007392-M01] - USF2 monoclonal antibody (M01), clone 5E9. Analysis of USF2 expression in different cell lines.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USF2 Antibody (5E9) [H00007392-M01] - USF2 monoclonal antibody (M01), clone 5E9. Analysis of USF2 expression in Hela S3 NE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USF2 Antibody (5E9) [H00007392-M01] - USF2 monoclonal antibody (M01), clone 5E9. Analysis of USF2 expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: USF2 Antibody (5E9) [H00007392-M01] - Detection limit for recombinant GST tagged USF2 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: USF2 Antibody (5E9) [H00007392-M01] - Analysis of monoclonal antibody to USF2 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USF2 Antibody (5E9) [H00007392-M01] - Analysis of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 1 ug/ml.
