PAB23725

antibody from Abnova Corporation

Targeting: NUTF2 NTF2, PP15

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23725

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23725, RRID:AB_11122002

Product name

NUTF2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NUTF2.

Antigen sequence

MGDKPIWEQIGSSFIQHYYQLFDNDRTQLGAIYID
ASCLTWEGQQFQGKAAIVEKLSSLPFQK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with NUTF2 polyclonal antibody (Cat # PAB23725).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with NUTF2 polyclonal antibody (Cat # PAB23725) at 1-4 ug/mL dilution shows positivity in cytoskeleton (microtubules).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human bronchus with NUTF2 polyclonal antibody (Cat # PAB23725) shows strong cytoplasmic and membranous positivity in respiratory epithelial cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)