AF4304

antibody from R&D Systems

Targeting: MAX bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4304

Provider

R&D Systems

Product name

Human Max Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Max in Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P61244


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Reversible linkage of two distinct small molecule inhibitors of Myc generates a dimeric inhibitor with improved potency that is active in myc over-expressing cancer cell lines.

Wanner J, Romashko D, Werner DS, May EW, Peng Y, Schulz R, Foreman KW, Russo S, Arnold LD, Pingle M, Bergstrom DE, Barany F, Thomson S
PloS one 2015;10(4):e0121793

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Max by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. Gels were loaded with 20 μg of cytoplasmic (Cyto) and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human Max Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4304) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Max was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.