12949-1-AP

antibody from Proteintech Group

Targeting: PDK4

Provider product page for 12949-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [62]
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Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 12949-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12949-1-AP, RRID:AB_2161499
Product name: PDK4 antibody
Antibody type: Polyclonal
Description: KD/KO validated PDK4 antibody (Cat. #12949-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PDK4 protein structure - 12949-1-AP shown in red.

Submitted references A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise.

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PloS one 2024;19(1):e0295964

Artemether regulates liver glycogen and lipid utilization through mitochondrial pyruvate oxidation in db/db mice.

Weng W, Shen L, Yu X, Yuan R, Shao M, Han P, Sun H
American journal of translational research 2024;16(1):27-38

AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine.

Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
Cell research 2024 Oct;34(10):683-706

Stevioside Ameliorates Palmitic Acid-Induced Abnormal Glucose Uptake via the PDK4/AMPK/TBC1D1 Pathway in C2C12 Myotubes.

Zhang C, Li S, Li L, Wang R, Luo S, Li G
Endocrinology, diabetes & metabolism 2024 May;7(3):e00482

12-O-deacetyl-phomoxanthone A inhibits ovarian tumor growth and metastasis by downregulating PDK4.

Yang C, Xing S, Wei X, Lu J, Zhao G, Ma X, Dai Z, Liang X, Huang W, Liu Y, Jiang X, Zhu D
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Jun;175:116736

Eicosapentaenoic acid increases proportion of type 1 muscle fibers through PPARδ and AMPK pathways in rats.

Komiya Y, Sakazaki Y, Goto T, Kawabata F, Suzuki T, Sato Y, Sawano S, Nakamura M, Tatsumi R, Ikeuchi Y, Arihara K, Mizunoya W
iScience 2024 Jun 21;27(6):109816

Dietary oleic acid intake increases the proportion of type 1 and 2X muscle fibers in mice.

Komiya Y, Iseki S, Ochiai M, Takahashi Y, Yokoyama I, Suzuki T, Tatsumi R, Sawano S, Mizunoya W, Arihara K
Scientific reports 2024 Jan 8;14(1):755

Enhancing aortic valve drug delivery with PAR2-targeting magnetic nano-cargoes for calcification alleviation.

Chen J, Ren T, Xie L, Hu H, Li X, Maitusong M, Zhou X, Hu W, Xu D, Qian Y, Cheng S, Yu K, Wang JA, Liu X
Nature communications 2024 Jan 16;15(1):557

Inhibition of Pyruvate Dehydrogenase Kinase 4 Protects Cardiomyocytes from lipopolysaccharide-Induced Mitochondrial Damage by Reducing Lactate Accumulation.

Chen T, Xie Q, Tan B, Yi Q, Xiang H, Wang R, Zhou Q, He B, Tian J, Zhu J, Xu H
Inflammation 2024 Aug;47(4):1356-1370

Monocarboxylate transporter 4 deficiency enhances high-intensity interval training-induced metabolic adaptations in skeletal muscle.

Tamura Y, Jee E, Kouzaki K, Kotani T, Nakazato K
The Journal of physiology 2024 Apr;602(7):1313-1340

A tryptophan-derived uremic metabolite/Ahr/Pdk4 axis governs skeletal muscle mitochondrial energetics in chronic kidney disease.

Thome T, Vugman NA, Stone LE, Wimberly K, Scali ST, Ryan TE
JCI insight 2024 Apr 23;9(10)

Inhibition of Pyruvate Dehydrogenase Kinase 4 Attenuates Myocardial and Mitochondrial Injury in Sepsis-Induced Cardiomyopathy.

Chen T, Ye L, Zhu J, Tan B, Yi Q, Sun Y, Xie Q, Xiang H, Wang R, Tian J, Xu H
The Journal of infectious diseases 2024 Apr 12;229(4):1178-1188

Identification of PDK4 as Hub Gene for Diabetic Nephropathy Using Co-Expression Network Analysis.

Han Y, Jin L, Wang L, Wei L, Tu C
Kidney & blood pressure research 2023;48(1):522-534

Prognostic value of autophagy-related genes based on single-cell RNA-sequencing in colorectal cancer.

Luo Y, Deng X, Liao W, Huang Y, Lu C
Frontiers in genetics 2023;14:1109683

PPAR/PDK4 pathway is involved in the anticancer effects of cGMP in pancreatic cancer.

Yamashita M, Kumazoe M, Onda H, Hiroi S, Shimada Y, Fujimura Y, Tachibana H
Biochemical and biophysical research communications 2023 Sep 10;672:154-160

PDK4 facilitates fibroblast functions and diabetic wound healing through regulation of HIF-1α protein stability and gene expression.

Ma Z, Mo R, Yang P, Ding Y, Zhang H, Dong Z, Chen Y, Tan Q
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Oct;37(10):e23215

Glucose Controls Glucagon Secretion by Regulating Fatty Acid Oxidation in Pancreatic α-Cells.

Armour SL, Frueh A, Chibalina MV, Dou H, Argemi-Muntadas L, Hamilton A, Katzilieris-Petras G, Carmeliet P, Davies B, Moritz T, Eliasson L, Rorsman P, Knudsen JG
Diabetes 2023 Oct 1;72(10):1446-1459

PDK4 rescues high-glucose-induced senescent fibroblasts and promotes diabetic wound healing through enhancing glycolysis and regulating YAP and JNK pathway.

Ma Z, Ding Y, Ding X, Mou H, Mo R, Tan Q
Cell death discovery 2023 Nov 25;9(1):424

Low-dose atorvastatin protects skeletal muscle mitochondria in high-fat diet-fed mice with mitochondrial autophagy inhibition and fusion enhancement.

Zheng P, Zhang Q, Ma W, Hu R, Gu Y, Bian Z, Yang D, Chen X, Wu H
European journal of pharmacology 2023 Nov 15;959:176085

Low-Carbohydrate Diet Modulates Glucose-Lipid Utilization in Skeletal Muscle of Diabetic Mice.

Li Y, Yang Z, Wang Y, Fan M, Nie C, Xue L, Wang L, Qian H
Nutrients 2023 Mar 21;15(6)

Docosahexaenoic Acid Alters Lipid Metabolism Processes via H3K9ac Epigenetic Modification in Dairy Goat.

Wu J, Luo J, He Q, Xia Y, Tian H, Zhu L, Li C, Loor JJ
Journal of agricultural and food chemistry 2023 Jun 7;71(22):8527-8539

METTL16 promotes glycolytic metabolism reprogramming and colorectal cancer progression.

Wei W, Zhang ZY, Shi B, Cai Y, Zhang HS, Sun CL, Fei YF, Zhong W, Zhang S, Wang C, He B, Jiang GM, Wang H
Journal of experimental & clinical cancer research : CR 2023 Jun 20;42(1):151

Acetate controls endothelial-to-mesenchymal transition.

Zhu X, Wang Y, Soaita I, Lee HW, Bae H, Boutagy N, Bostwick A, Zhang RM, Bowman C, Xu Y, Trefely S, Chen Y, Qin L, Sessa W, Tellides G, Jang C, Snyder NW, Yu L, Arany Z, Simons M
Cell metabolism 2023 Jul 11;35(7):1163-1178.e10

1,5-Anhydroglucitol promotes pre-B acute lymphocytic leukemia progression by driving glycolysis and reactive oxygen species formation.

Zhu H, Ma H, Dong N, Wu M, Li D, Liu L, Shi Q, Ju X
BMC cancer 2023 Feb 6;23(1):122

Astrocytic FoxO1 in the hypothalamus regulates metabolic homeostasis by coordinating neuropeptide Y neuron activity.

Doan KV, Tran LT, Yang DJ, Ha TTA, Mai TD, Kim SK, DePinho RA, Shin DM, Choi YH, Kim KW
Glia 2023 Dec;71(12):2735-2752

Distinct Transcriptional Responses of Skeletal Muscle to Short-Term Cold Exposure in Tibetan Pigs and Bama Pigs.

Yang C, Cao C, Liu J, Zhao Y, Pan J, Tao C, Wang Y
International journal of molecular sciences 2023 Apr 18;24(8)

MCC950, a Selective NLRP3 Inhibitor, Attenuates Adverse Cardiac Remodeling Following Heart Failure Through Improving the Cardiometabolic Dysfunction in Obese Mice.

Wang M, Zhao M, Yu J, Xu Y, Zhang J, Liu J, Zheng Z, Ye J, Wang Z, Ye D, Feng Y, Xu S, Pan W, Wei C, Wan J
Frontiers in cardiovascular medicine 2022;9:727474

Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.

Zhang H, Yan M, Liu T, Wei P, Chai N, Li L, Wang J, Yu X, Lin Y, Qiu B, Zhao Y
Frontiers in cell and developmental biology 2022;10:840389

Ginsenoside Rg3 ameliorates myocardial glucose metabolism and insulin resistance via activating the AMPK signaling pathway.

Ni J, Liu Z, Jiang M, Li L, Deng J, Wang X, Su J, Zhu Y, He F, Mao J, Gao X, Fan G
Journal of ginseng research 2022 Mar;46(2):235-247

Exercise-induced histone H3 trimethylation at lysine 27 facilitates the adaptation of skeletal muscle to exercise in mice.

Shimizu J, Kawano F
The Journal of physiology 2022 Jul;600(14):3331-3353

Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice.

Zhu B, Zhong W, Cao X, Pan G, Xu M, Zheng J, Chen H, Feng X, Luo C, Lu C, Xiao J, Lin W, Lai C, Li M, Du X, Yi Q, Yan D
Science translational medicine 2022 Jan 26;14(629):eabh2548

Inhibition of pyruvate dehydrogenase kinase improves carbohydrate utilization in Nile tilapia by regulating PDK2/4-PDHE1α axis and insulin sensitivity.

Luo Y, Zhou W, Li R, Limbu SM, Qiao F, Chen L, Zhang M, Du ZY
Animal nutrition (Zhongguo xu mu shou yi xue hui) 2022 Dec;11:25-37

Prostaglandin E1 attenuates AngII-induced cardiac hypertrophy via EP3 receptor activation and Netrin-1upregulation.

Shen Y, Wang X, Yuan R, Pan X, Yang X, Cai J, Li Y, Yin A, Xiao Q, Ji Q, Li Y, He B, Shen L
Journal of molecular and cellular cardiology 2021 Oct;159:91-104

Sex differences in metabolic pathways are regulated by Pfkfb3 and Pdk4 expression in rodent muscle.

Christianto A, Baba T, Takahashi F, Inui K, Inoue M, Suyama M, Ono Y, Ohkawa Y, Morohashi KI
Communications biology 2021 Nov 4;4(1):1264

Effect of Notch pathway on lipid accumulation induced by mono-2-ethylhexyl phthalate on 3T3-L1 cells.

Qi W, Xu Q, Xu Y, Wang Z, Yang L, Guo S, Shi Y, Zhao T, Zhou L, Ye L
Ecotoxicology and environmental safety 2021 Jan 15;208:111472

PDK4 dictates metabolic resistance to ferroptosis by suppressing pyruvate oxidation and fatty acid synthesis.

Song X, Liu J, Kuang F, Chen X, Zeh HJ 3rd, Kang R, Kroemer G, Xie Y, Tang D
Cell reports 2021 Feb 23;34(8):108767

Effects of Dietary Fat Restriction on Endurance Training-induced Metabolic Adaptations in Rat Skeletal Muscle.

Karasawa T, Kondo S, Fukazawa A, Koike A, Tsutsui M, Terada S
Journal of oleo science 2021 Feb 1;70(2):253-262

Dietary Olive Oil Intake Improves Running Endurance with Intramuscular Triacylglycerol Accumulation in Mice.

Komiya Y, Sugiyama M, Ochiai M, Osawa N, Adachi Y, Iseki S, Arihara K
Nutrients 2021 Apr 1;13(4)

Difference in pyruvic acid metabolism between neonatal and adult mouse lungs exposed to hyperoxia.

Tanaka K, Watanabe T, Ozawa J, Ito M, Nagano N, Arai Y, Miyake F, Matsumura S, Kobayashi S, Itakura R, Namba F
PloS one 2020;15(9):e0238604

Loss of PDK4 expression promotes proliferation, tumorigenicity, motility and invasion of hepatocellular carcinoma cells.

Qin YJ, Lin TY, Lin XL, Liu Y, Zhao WT, Li XY, Lian M, Chen HW, Li YL, Zhang XL, Xiao D, Jia JS, Sun Y
Journal of Cancer 2020;11(15):4397-4405

Mitochondrial Utilization of Competing Fuels Is Altered in Insulin Resistant Skeletal Muscle of Non-obese Rats (Goto-Kakizaki).

Lai N, Fealy CE, Kummitha CM, Cabras S, Kirwan JP, Hoppel CL
Frontiers in physiology 2020;11:677

ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Zhang X, Dong Y, Zhao M, Ding L, Yang X, Jing Y, Song Y, Chen S, Hu Q, Ni Y
Theranostics 2020;10(26):12044-12059

Spexin protects cardiomyocytes from hypoxia-induced metabolic and mitochondrial dysfunction.

Liu Y, Sun L, Zheng L, Su M, Liu H, Wei Y, Li D, Wang Y, Dai C, Gong Y, Zhao C, Li Y
Naunyn-Schmiedeberg's archives of pharmacology 2020 Jan;393(1):25-33

Succinate dehydrogenase deficiency in a chromaffin cell model retains metabolic fitness through the maintenance of mitochondrial NADH oxidoreductase function.

Kľučková K, Thakker A, Vettore L, Escribano-Gonzalez C, Hindshaw RL, Tearle JLE, Goncalves J, Kaul B, Lavery GG, Favier J, Tennant DA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jan;34(1):303-315

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Wang L, Yu P, Zhou B, Song J, Li Z, Zhang M, Guo G, Wang Y, Chen X, Han L, Hu S
Nature cell biology 2020 Jan;22(1):108-119

Thiamine mimetics sulbutiamine and benfotiamine as a nutraceutical approach to anticancer therapy.

Jonus HC, Byrnes CC, Kim J, Valle ML, Bartlett MG, Said HM, Zastre JA
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Jan;121:109648

Effects of di (2-ethylhexyl) phthalate and high-fat diet on lipid metabolism in rats by JAK2/STAT5.

Zhang Y, Zhou L, Zhang Z, Xu Q, Han X, Zhao Y, Song X, Zhao T, Ye L
Environmental science and pollution research international 2020 Feb;27(4):3837-3848

Metabolomics reveals metabolite changes of patients with pulmonary arterial hypertension in China.

Chen C, Luo F, Wu P, Huang Y, Das A, Chen S, Chen J, Hu X, Li F, Fang Z, Zhou S
Journal of cellular and molecular medicine 2020 Feb;24(4):2484-2496

STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.

Oberhuber M, Pecoraro M, Rusz M, Oberhuber G, Wieselberg M, Haslinger P, Gurnhofer E, Schlederer M, Limberger T, Lagger S, Pencik J, Kodajova P, Högler S, Stockmaier G, Grund-Gröschke S, Aberger F, Bolis M, Theurillat JP, Wiebringhaus R, Weiss T, Haitel A, Brehme M, Wadsak W, Griss J, Mohr T, Hofer A, Jäger A, Pollheimer J, Egger G, Koellensperger G, Mann M, Hantusch B, Kenner L
Molecular systems biology 2020 Apr;16(4):e9247

Effects of a Ketogenic Diet Containing Medium-Chain Triglycerides and Endurance Training on Metabolic Enzyme Adaptations in Rat Skeletal Muscle.

Fukazawa A, Koike A, Karasawa T, Tsutsui M, Kondo S, Terada S
Nutrients 2020 Apr 30;12(5)

Overexpression of PDK4 is associated with cell proliferation, drug resistance and poor prognosis in ovarian cancer.

Wang J, Qian Y, Gao M
Cancer management and research 2019;11:251-262

Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells.

Zhang Y, Wang S, Zhao T, Yang L, Guo S, Shi Y, Zhang X, Zhou L, Ye L
Ecotoxicology and environmental safety 2019 Nov 30;184:109611

Geniposide Improves Glucose Homeostasis via Regulating FoxO1/PDK4 in Skeletal Muscle.

Li Y, Pan H, Zhang X, Wang H, Liu S, Zhang H, Qian H, Wang L, Ying H
Journal of agricultural and food chemistry 2019 Apr 24;67(16):4483-4492

Retrodifferentiation of Human Tumor Hepatocytes to Stem Cells Leads to Metabolic Reprogramming and Chemoresistance.

Fekir K, Dubois-Pot-Schneider H, Désert R, Daniel Y, Glaise D, Rauch C, Morel F, Fromenty B, Musso O, Cabillic F, Corlu A
Cancer research 2019 Apr 15;79(8):1869-1883

Effects of functional β-glucan on proliferation, differentiation, metabolism and its anti-fibrosis properties in muscle cells.

Li Y, Fan Y, Pan H, Qian H, Qi X, Wu G, Zhang H, Xu M, Rao Z, Wang L, Ying H
International journal of biological macromolecules 2018 Oct 1;117:287-293

AMPK/GSK3β/β-catenin cascade-triggered overexpression of CEMIP promotes migration and invasion in anoikis-resistant prostate cancer cells by enhancing metabolic reprogramming.

Zhang P, Song Y, Sun Y, Li X, Chen L, Yang L, Xing Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jul;32(7):3924-3935

Down-regulation of PDK4 is Critical for the Switch of Carbohydrate Catabolism during Syncytialization of Human Placental Trophoblasts.

Liu X, Zuo R, Bao Y, Qu X, Sun K, Ying H
Scientific reports 2017 Aug 16;7(1):8474

Enhancement of Glucose Metabolism via PGC-1α Participates in the Cardioprotection of Chronic Intermittent Hypobaric Hypoxia.

Li X, Liu Y, Ma H, Guan Y, Cao Y, Tian Y, Zhang Y
Frontiers in physiology 2016;7:219

MiR-155 Enhances Insulin Sensitivity by Coordinated Regulation of Multiple Genes in Mice.

Lin X, Qin Y, Jia J, Lin T, Lin X, Chen L, Zeng H, Han Y, Wu L, Huang S, Wang M, Huang S, Xie R, Liang L, Liu Y, Liu R, Zhang T, Li J, Wang S, Sun P, Huang W, Yao K, Xu K, Du T, Xiao D
PLoS genetics 2016 Oct;12(10):e1006308

Effects of neonatal dexamethasone administration on cardiac recovery ability under ischemia-reperfusion in 24-wk-old rats.

Jiang X, Ma H, Li C, Cao Y, Wang Y, Zhang Y, Liu Y
Pediatric research 2016 Jul;80(1):128-35

AMP-Activated Protein Kinase Interacts with the Peroxisome Proliferator-Activated Receptor Delta to Induce Genes Affecting Fatty Acid Oxidation in Human Macrophages.

Kemmerer M, Finkernagel F, Cavalcante MF, Abdalla DS, Müller R, Brüne B, Namgaladze D
PloS one 2015;10(6):e0130893

PPARγ isoforms differentially regulate metabolic networks to mediate mouse prostatic epithelial differentiation.

Strand DW, Jiang M, Murphy TA, Yi Y, Konvinse KC, Franco OE, Wang Y, Young JD, Hayward SW
Cell death & disease 2012 Aug 9;3(8):e361

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse heart tissue were subjected to SDS PAGE followed by western blot with 12949-1-AP(PDK4 antibody) at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 12949-1-AP(PDK4 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The PDK4 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PDK4. This antibody recognizes human,mouse,rat antigen. The PDK4 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 12949-1-AP.pdf

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