Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA302046 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA302046, RRID:AB_2161611
- Product name
- Rabbit Polyclonal Antibody against PDK4 (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Antibody against PDK4 (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PDK4
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- The PDK4 Antibody (C-term) (Cat# TA302046) is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- PDK4 Antibody (C396) (Cat. #TA302046) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PDK4 antibody detected the PDK4 protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- The PDK4 Antibody (C-term) (Cat# TA302046) is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of HeLa cells stained with PDK4 (C-term) antibody. HeLa cells were incubated with TA302046 PDK4 (C-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the PDK4 mainly to the cytoplasm.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term) (Cat. #TA302046), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human Adrenal tissue reacted with PDK4 Antibody (C-term) (Cat. #TA302046), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
