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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- F50055 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PDK4 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 382-410 from the human protein was used as the immunogen for this PDK4 antibody.
- Description
- Purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the PDK4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of PDK4 antibody and mouse skeletal muscle tissue lysate. Predicted molecular weight ~46 kDa
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of HeLa cells stained with PDK4 antibody. For secondary Ab, Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). PDK4 is localized to the cytoplasm.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human adrenal tissue stained with PDK4 antibody
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human skeletal muscle stained with PDK4 antibody
