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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PAB4564 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4564, RRID:AB_1578573
- Product name
- PDK4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PDK4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Regulation of the PDK4 isozyme by the Rb-E2F1 complex.
CD36-dependent regulation of muscle FoxO1 and PDK4 in the PPAR delta/beta-mediated adaptation to metabolic stress.
The STAT5A-mediated induction of pyruvate dehydrogenase kinase 4 expression by prolactin or growth hormone in adipocytes.
Hsieh MC, Das D, Sambandam N, Zhang MQ, Nahlé Z
The Journal of biological chemistry 2008 Oct 10;283(41):27410-7
The Journal of biological chemistry 2008 Oct 10;283(41):27410-7
CD36-dependent regulation of muscle FoxO1 and PDK4 in the PPAR delta/beta-mediated adaptation to metabolic stress.
Nahlé Z, Hsieh M, Pietka T, Coburn CT, Grimaldi PA, Zhang MQ, Das D, Abumrad NA
The Journal of biological chemistry 2008 May 23;283(21):14317-26
The Journal of biological chemistry 2008 May 23;283(21):14317-26
The STAT5A-mediated induction of pyruvate dehydrogenase kinase 4 expression by prolactin or growth hormone in adipocytes.
White UA, Coulter AA, Miles TK, Stephens JM
Diabetes 2007 Jun;56(6):1623-9
Diabetes 2007 Jun;56(6):1623-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PDK4 (arrow) using PDK4 polyclonal antibody (Cat # PAB4564). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK4 gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PDK4 polyclonal antibody (Cat # PAB4564) is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skeletal musclereacted with PDK4 polyclonal antibody (Cat # PAB4564), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human adrenalreacted with PDK4 polyclonal antibody (Cat # PAB4564), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
