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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4565 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4565, RRID:AB_1578574
- Product name
- PDK4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PDK4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients.
Downregulation of metabolic gene expression in failing human heart before and after mechanical unloading.
Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human.
Rosa G, Di Rocco P, Manco M, Greco AV, Castagneto M, Vidal H, Mingrone G
Obesity research 2003 Feb;11(2):176-82
Obesity research 2003 Feb;11(2):176-82
Downregulation of metabolic gene expression in failing human heart before and after mechanical unloading.
Razeghi P, Young ME, Ying J, Depre C, Uray IP, Kolesar J, Shipley GL, Moravec CS, Davies PJ, Frazier OH, Taegtmeyer H
Cardiology 2002;97(4):203-9
Cardiology 2002;97(4):203-9
Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human.
Rowles J, Scherer SW, Xi T, Majer M, Nickle DC, Rommens JM, Popov KM, Harris RA, Riebow NL, Xia J, Tsui LC, Bogardus C, Prochazka M
The Journal of biological chemistry 1996 Sep 13;271(37):22376-82
The Journal of biological chemistry 1996 Sep 13;271(37):22376-82
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PDK4 polyclonal antibody (Cat # PAB4565) in CEM cell line lysates (35 ug/lane). PDK4 (arrow) was detected using the purified PDK4 polyclonal antibody (Cat # PAB4565) (1 : 60 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 polyclonal antibody (Cat # PAB4565) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
