PAB22272

antibody from Abnova Corporation

Targeting: CCT4 Cctd

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22272

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22272, RRID:AB_10962821

Product name

CCT4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CCT4.

Antigen sequence

GSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGG
APEIELALRLTEYSRTLSGMESYCVRAFADAMEVI
PSTLAENAGLNPISTVTELRNRHAQGEKTAGINVR
KGGISNILEELVVQPLLVSVSALTLATETVRSILK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with CCT4 polyclonal antibody (Cat # PAB22272) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with CCT4 polyclonal antibody (Cat # PAB22272) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with CCT4 polyclonal antibody (Cat # PAB22272) shows strong cytoplasmic positivity in cells in seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)