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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2035 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2035, RRID:AB_1578458
- Product name
- PBK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PBK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Protein expression of PDZ-binding kinase is up-regulated in hematologic malignancies and strongly down-regulated during terminal differentiation of HL-60 leukemic cells.
PBK/TOPK is a novel mitotic kinase which is upregulated in Burkitt's lymphoma and other highly proliferative malignant cells.
PDZ-binding kinase participates in spermatogenesis.
Nandi A, Tidwell M, Karp J, Rapoport AP
Blood cells, molecules & diseases 2004 Jan-Feb;32(1):240-5
Blood cells, molecules & diseases 2004 Jan-Feb;32(1):240-5
PBK/TOPK is a novel mitotic kinase which is upregulated in Burkitt's lymphoma and other highly proliferative malignant cells.
Simons-Evelyn M, Bailey-Dell K, Toretsky JA, Ross DD, Fenton R, Kalvakolanu D, Rapoport AP
Blood cells, molecules & diseases 2001 Sep-Oct;27(5):825-9
Blood cells, molecules & diseases 2001 Sep-Oct;27(5):825-9
PDZ-binding kinase participates in spermatogenesis.
Zhao S, Dai J, Zhao W, Xia F, Zhou Z, Wang W, Gu S, Ying K, Xie Y, Mao Y
The international journal of biochemistry & cell biology 2001 Jun;33(6):631-6
The international journal of biochemistry & cell biology 2001 Jun;33(6):631-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PBK (arrow) using PBK polyclonal antibody (Cat # PAB2035). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK polyclonal antibody (Cat # PAB2035) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
