NBP1-05950

antibody from Novus Biologicals

Targeting: LCAT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

NBP1-05950

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-05950, RRID:AB_1556170

Product name

Rabbit Polyclonal LCAT Antibody

Antibody type

Polyclonal

Description

Unpurified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins.

Hirsch-Reinshagen V, Donkin J, Stukas S, Chan J, Wilkinson A, Fan J, Parks JS, Kuivenhoven JA, Lütjohann D, Pritchard H, Wellington CL
Journal of lipid research 2009 May;50(5):885-93

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Initial interaction of apoA-I with ABCA1 impacts in vivo metabolic fate of nascent HDL.

Mulya A, Lee JY, Gebre AK, Boudyguina EY, Chung SK, Smith TL, Colvin PL, Jiang XC, Parks JS
Journal of lipid research 2008 Nov;49(11):2390-401

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Functional LCAT deficiency in human apolipoprotein A-I transgenic, SR-BI knockout mice.

Lee JY, Badeau RM, Mulya A, Boudyguina E, Gebre AK, Smith TL, Parks JS
Journal of lipid research 2007 May;48(5):1052-61

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LCAT Antibody [NBP1-05950] - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot analysis using rabbit antiserum against mouse LCAT.