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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-121105 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LDB3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: HL-60, MCF7, U-937, Raji, BT-474, Mouse skeletal muscle, Rat skeletal muscle. The target is usually found in the following locations: Cell projection, Cytoplasm, Z line, cytoskeleton, myofibril, perinuclear region, pseudopodium, sarcomere. Immunogen sequence: MSYSVTLTGP GPWGFRLQGG KDFNMPLTIS RITPGSKAAQ SQLSQGDLVV AIDGVNTDTM THLEAQNKIK SASYNLSLTL QKSKRPIPIS TTAPPVQTPL PVIPHQKVVV NSPANADYQE RFNPSALKDS ALSTHKPIEV KGLGGKATII HAQYNTPISM YSQDAIMDAI AGQAQAQGSD FSGSLPIKDL AVDSASPVYQ AVIKSQNKPE DEADEWARRS SNLQSRSFRI LAQMTGTEFM QDPDEEALRR SRERFETERN SPRFAKLRNW HHGLSAQILN VKS
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.12 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LDB3 in various cell lines using a LDB3 Polyclonal antibody (Product # PA5-121105) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of LDB3 in human placenta cells using a LDB3 Polyclonal antibody (Product # PA5-121105). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of LDB3 in human placenta cells using a LDB3 Polyclonal antibody (Product # PA5-121105). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of LDB3 in human placenta cells using a LDB3 Polyclonal antibody (Product # PA5-121105). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LDB3 in mouse stomach using a Polyclonal antibody (Product # PA5-121105) at a dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..
