15904-1-AP
antibody from Invitrogen Antibodies
Targeting: MDH1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 15904-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MDH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSEPIRVLV TGAAGQIAYS LLYSIGNGSV FGKDQPIILV LLDITPMMGV LDGVLMELQD CALPLLKDVI ATDKEDVAFK DLDVAILVGS MPRREGMERK DLLKANVKIF KSQGAALDKY AKKSVKVIVV GNPANTNCLT ASKSAPSIPK ENFSCLTRLD HNRAKAQIAL KLGVTANDVK NVIIWGNHSS TQYPDVNHAK VKLQGKEVGV YEALKDDSWL KGEFVTTVQQ RGAAVIKARK LSSAMSAAKA ICDHVRDIWF GTPEGEFVSM GVISDGNSYG VPDDLLYSFP VVIKNKTWKF VEGLPINDFS REKMDLTAKE LTEEKESAFE FLSSA (1-334 aa encoded by BC001484)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.17 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of MDH1 antibody (15904-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human spleen tissue were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HL-60 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using MDH1 antibody 15904-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP ( MDH1 Antibody) at dilution of 1:100 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP ( MDH1 Antibody) at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug MDH1 antibody (15904-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-MDH1 (IP:15904-1-AP, 4ug; Detection:15904-1-AP 1:1000) with mouse liver tissue lysate 6400ug.