PA5-29476

antibody from Invitrogen Antibodies

Targeting: ASB2 ASB-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29476

Provider

Invitrogen Antibodies

Product name

ASB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HL-60.

Concentration

1 mg/mL

References

Submitted references

ASB2 is a direct target of FLI1 that sustains NF-κB pathway activation in germinal center-derived diffuse large B-cell lymphoma.

Sartori G, Napoli S, Cascione L, Chung EYL, Priebe V, Arribas AJ, Mensah AA, Dall'Angelo M, Falzarano C, Barnabei L, Forcato M, Rinaldi A, Bicciato S, Thome M, Bertoni F
Journal of experimental & clinical cancer research : CR 2021 Nov 11;40(1):357

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASB2 using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an ASB2 polyclonal antibody (Product # PA5-29476) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ASB2 was performed by separating 30 µg of whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ASB2 Polyclonal Antibody (Product # PA5-29476) at a dilution of 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ASB2 Polyclonal Antibody detects ASB2 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ASB2 Polyclonal Antibody (Product # PA5-29476) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 ASB2 downregulation in DLBCL cell lines harvested 72 h after nucleofection. A MTT assay for DLBCL cell lines nucleofected with either 500 nM control (CNT) siRNA, FLI1 siRNA or ASB2 siRNA. B Normalized (to GAPDH) relative mRNA expression of ASB2 from CNT siRNA and ASB2 siRNA treated cells. C Immunoblot and its quantification showing protein expression of ASB2 and IkappaBalpha in DLBCL CNT siRNA, FLI1 siRNA and ASB2 siRNA treated cells. Mouse monoclonal alpha-GAPDH was used as loading control. For each figure two replicates were performed for each cell line

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 ASB2 downregulation in DLBCL cell lines harvested 48 h after nucleofection. A Immunoblots for NF-kappaB related genes after ASB2 and FLI1 downregulation and B quantification of protein bands. C Schema of the proposed mechanism of FLI1 transcriptional regulation of NF-kappaB1 and ASB2 genes, that respectively contribute to the activation of the classical and alternative NF-kappaB pathway. For each figure two replicates were performed for each cell line