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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP53818PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53818PU-N, RRID:AB_11146457
- Product name
- anti SCUBE2 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 258-288 amino acids from the Central region of Human SCUBE2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of SCUBE2 Antibody (Center) Cat.-No AP53818PU-N in MDA-MB453,ZR-75-1 cell line lysates (35 µg/lane). This demonstrates the SCUBE2 antibody detected the SCUBE2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal Immunofluorescent analysis of SCUBE2 Antibody (Center) Cat.-No AP53818PU-N with ZR-75-1 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human breast carcinoma stained with SCUBE2 Antibody (Center) Cat.-No AP53818PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SCUBE2 Antibody (Center N266) for immunohistochemistry. Clinical relevance has not been evaluated.
