Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB3208 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3208, RRID:AB_10550763
- Product name
- CFL1 monoclonal antibody, clone AT1C1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant CFL1.
- Isotype
- IgG
- Antibody clone number
- AT1C1
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mapping the ADF/cofilin binding site on monomeric actin by competitive cross-linking and peptide array: evidence for a second binding site on monomeric actin.
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization.
Mannherz HG, Ballweber E, Galla M, Villard S, Granier C, Steegborn C, Schmidtmann A, Jaquet K, Pope B, Weeds AG
Journal of molecular biology 2007 Feb 23;366(3):745-55
Journal of molecular biology 2007 Feb 23;366(3):745-55
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M
Cell 2006 Nov 3;127(3):635-48
Cell 2006 Nov 3;127(3):635-48
Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization.
Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K
Nature 1998 Jun 25;393(6687):809-12
Nature 1998 Jun 25;393(6687):809-12
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- The lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with CFL1 monoclonal antibody, clone AT1C1 (Cat # MAB3208 ; 1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.