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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- OAAB16052 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#OAAB16052, RRID:AB_2630495
- Product name
- HER4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Phospho-HER4-Y1162 Antibody (cat# OAAB16052) was used to detect Phospho-HER4-Y1162 in FG pancreatic carcinoma cell line lysate. FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min Phospho-HER4-Y1162 Antibody (cat# OAAB16052) was used at 1:750 in 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Phospho-HER4-Y1162 Antibody(Cat# OAAB16052) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red) .DAPI was used to stain the cell nuclear (blue) .
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
