GTX80811

antibody from GeneTex

Targeting: ERBB4 ALS19, HER4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX80811

Provider

GeneTex

Proper citation

GeneTex Cat#GTX80811, RRID:AB_625545

Product name

Her4 / ErbB4 antibody [HFR1]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of MDA-MB-453 and T47D lysates (25 ug per lane) using ErbB4 antibody [HFR1] at a dilution of 1:300.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of NIH-3T3 cells with (right) or without (left) ErbB4 antibody [HFR1] at a dilution of 1:50 (green). Nuclei were stained with DAPI (blue). Images were taken at a magnification of 60x.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of A431 cells with (right) or without (left) ErbB4 antibody [HFR1] at a dilution of 1:50 (green). Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with DAPI (blue). Images were taken at a magnification of 60x.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of SK-BR-3 cells with (right) or without (left) ErbB4 antibody [HFR1] at a dilution of 1:50 (green). Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with DAPI (blue). Images were taken at a magnification of 60x.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of human heart tissue with (left) or without (right) ErbB4 antibody [HFR1] at a dilution of 1:200. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of human pancreas tissue with (left) or without (right) ErbB4 antibody [HFR1] at a dilution of 1:200. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of human breast carcinoma with (left) or without (right) ErbB4 antibody [HFR1] at a dilution of 1:20. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FACS analysis of MCF-7 cells with ErbB4 antibody [HFR1] at a dilution of 1ug/test (green) or an isotype control (blue).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FACS analysis of HeLa cells with ErbB4 antibody [HFR1] at a dilution of 1ug/test (green) or an isotype control (blue).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FACS analysis of NIH-3T3 cells with ErbB4 antibody [HFR1] at a dilution of 0.5ug/test (green) or an isotype control (blue).