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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ARG55922 - Provider product page
- Provider
- Arigo
- Product name
- anti-ERBB4 / HER4 phospho (Tyr1162) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated phosphospecific peptide around Tyr1162 of Human ERBB4 / HER4
- Description
- Purification with Protein G and phospho-specific peptide, the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: FG pancreatic carcinoma cell line lysate treated with or without EGF (50 ng/ml) for 15 min, stained with ARG55922 anti-ERBB4 / HER4 phospho (Tyr1162) antibody at 1:750 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: MCF-7 cell stained with ARG55922 anti-ERBB4 / HER4 phospho (Tyr1162) antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded Human cancer tissue stained with ARG55922 anti-ERBB4 / HER4 phospho (Tyr1162) antibody.
