NB600-853

antibody from Novus Biologicals

Targeting: GLUD1 GDH, GLUD

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NB600-853

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-853, RRID:AB_10002587

Product name

Rabbit Polyclonal Glutamate Dehydrogenase Antibody

Antibody type

Polyclonal

Description

Delipidation and Defibrination.

Reactivity

Human, Bovine

Host

Rabbit

Vial size

0.2 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Unique metabolic features of pancreatic cancer stroma: relevance to the tumor compartment, prognosis, and invasive potential.

Knudsen ES, Balaji U, Freinkman E, McCue P, Witkiewicz AK
Oncotarget 2016 Nov 29;7(48):78396-78411

---

Glutamine oxidation maintains the TCA cycle and cell survival during impaired mitochondrial pyruvate transport.

Yang C, Ko B, Hensley CT, Jiang L, Wasti AT, Kim J, Sudderth J, Calvaruso MA, Lumata L, Mitsche M, Rutter J, Merritt ME, DeBerardinis RJ
Molecular cell 2014 Nov 6;56(3):414-24

---

Transgenic expression of Glud1 (glutamate dehydrogenase 1) in neurons: in vivo model of enhanced glutamate release, altered synaptic plasticity, and selective neuronal vulnerability.

Bao X, Pal R, Hascup KN, Wang Y, Wang WT, Xu W, Hui D, Agbas A, Wang X, Michaelis ML, Choi IY, Belousov AB, Gerhardt GA, Michaelis EK
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Nov 4;29(44):13929-44

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Glutamate Dehydrogenase Antibody [NB600-853] - Analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of 62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose. The blot was incubated with a 1:1,000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature.