H00030008-M19

antibody from Abnova Corporation

Targeting: EFEMP2 FBLN4, UPH1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00030008-M19

Provider

Abnova Corporation

Product name

EFEMP2 monoclonal antibody (M19), clone 2C8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant EFEMP2.

Antigen sequence

SPQDSEEPDSYTECTDGYEWDPDSQHCRDVNECLT
IPEACKGEMKCINHYGGYLCLPRSAAVINDLHGEG
PPPPVPPAQHPNPCPPGYEPDDQDSCVDVDECAQA
LHDCRPSQDCHNLPGSYQCTCPDGYRKIGPECVDI
DECRYRYCQHRCVNLPGSFRCQCEPGFQLGPNNRS
CVDVNECDMGAPCEQRCFNSYGTFLCRCHQGYELH
RDGFSCSDIDECSYSSYLCQYRCVNEPGRFSCHCP
QGYQLLATRLCQDIDECESGAHQCSEAQTCVNFHG
GYRCVDTNRCVEPYIQVSENRCLCPASNPLCREQP
SSIVHRYMTITSERSVPADVFQIQATSVYPGAYNA
FQIRAGNSQGDFYIRQINNVSAMLVLARPVTGPRE
YVLDLEMVTMNSLMSYRASSVLRLTVFVGAYTF


Isotype

IgG

Antibody clone number

2C8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of EFEMP2 expression in transfected 293T cell line by EFEMP2 monoclonal antibody (M01), clone 2C8.Lane 1: EFEMP2 transfected lysate(49.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EFEMP2 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of EFEMP2 transfected lysate using anti-EFEMP2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with EFEMP2 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol