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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00006301-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006301-M01, RRID:AB_436972
- Product name
- SARS monoclonal antibody (M01), clone 1H4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SARS.
- Antigen sequence
MVLDLDLFRVDKGGDPALIRETQEKRFKDPGLVDQ
LVKADSEWRRCRFRADNLNKLKNLCSKTIGEKMKK
KEPVGDDESVPENVLSFDDLTADALANLKVSQIKK
VRLLIDEAILKCDAERIKLEAERFENLREIGNLLH
PSVPISNDEDVDNKVERIWGDCTVRKKYSHVDLVV
MVDGFEGEKGAVVAGSRGYFLKGVLVFLEQALIQY
ALRTLGSRGYIPIYTPFFMRKEVMQEVAQLSQFDE
ELYKVIGKGSEKSDDNSYDEKYLIATSEQPIAALH
RDEWLRPEDLPIKYAGLSTCFRQEVGSHGRDTRGI
FRVHQFEKIEQFVYSSPHDNKSWEMFEEMITTAEE
FYQSLGIPYHIVNIVSGSLNHAASKKLDLEAWFPG
SGAFRELVSCSNCTDYQARRLRIRYGQTKKMMDKV
EFVHMLNATMCATTRTICAILENYQTEKGITVPEK
LKEFMPPGLQELIPFVKPAPIEQEPSKKQKKQHEG
SKKKAAARDVTLENRLQNMEVTDA- Isotype
- IgG
- Antibody clone number
- 1H4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references tRNA synthetase counteracts c-Myc to develop functional vasculature.
MiR-1 and miR-206 target different genes to have opposing roles during angiogenesis in zebrafish embryos.
Shi Y, Xu X, Zhang Q, Fu G, Mo Z, Wang GS, Kishi S, Yang XL
eLife 2014 Jun 17;3:e02349
eLife 2014 Jun 17;3:e02349
MiR-1 and miR-206 target different genes to have opposing roles during angiogenesis in zebrafish embryos.
Lin CY, Lee HC, Fu CY, Ding YY, Chen JS, Lee MH, Huang WJ, Tsai HJ
Nature communications 2013;4:2829
Nature communications 2013;4:2829
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SARS monoclonal antibody (M01), clone 1H4 Western Blot analysis of SARS expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SARS expression in transfected 293T cell line by SARS monoclonal antibody (M01), clone 1H4.Lane 1: SARS transfected lysate(58.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SARS is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to SARS on HeLa cell. [antibody concentration 25 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to SARS on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
