- UM800081 - OriGene LGALS3 antibody

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Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-LGALS3 monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500) Dilution: 1:500
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunofluorescent staining of HeLa cells using anti-LGALS3 mouse monoclonal antibody (UM800081, green, 1:50). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LGALS3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800081)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800081)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human tonsil using anti-LGALS3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800081)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti- Galectin 3 mouse monoclonal antibody at 1:400 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800081 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110C for 3 min using pressure chamber/cooker. The tumor cells shows strong membrane and cytoplasmic staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human pancreas using anti- Galectin 3 mouse monoclonal antibody at 1:400 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800081 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110C for 3 min using pressure chamber/cooker. The show weak cytoplasmic staining in pacreatic cells and strong cytoplasmic and membraneous staining in interlobular duct cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded carcinoma of human thyroid using anti- Galectin 3 mouse monoclonal antibody at 1:400 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800081 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110C for 3 min using pressure chamber/cooker. The tumor cells shows strong membrane and cytoplasmic staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-LGALS3 mouse monoclonal antibody (UM800081). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
Validation comment: 10K-CHIP

UM800081