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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-C790343 - Provider product page
- Provider
- LSBio
- Product name
- LGALS3 / Galectin 3 Antibody (clone OTI8E3, Carrier-free) LS-C790343
- Antibody type
- Monoclonal
- Description
- Purified from ascites.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI8E3
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY LGALS3. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3. (1:2000)
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Equivalent amounts of cell lysates and LGALS3-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-LGALS3 monoclonal antibodyThen the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control. (1:500)
