LS-C790343

LGALS3 antibody from LifeSpan BioSciences, Inc.

GALIG, LGALS2, MAC-2

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Recommended by provider

Provider product page for LS-C790343

Antibody Data

Product number

LS-C790343

Provider

LifeSpan BioSciences, Inc.

Product name

LGALS3 / Galectin 3 Antibody (clone OTI8E3, Carrier-free) LS-C790343

Provider product page

Antibody type

Monoclonal

Description

Purified from ascites.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI8E3

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LifeSpan BioSciences, Inc. (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY LGALS3. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3. (1:2000)

Supportive validation

Submitted by

LifeSpan BioSciences, Inc. (provider)

Main image

Experimental details

Equivalent amounts of cell lysates and LGALS3-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-LGALS3 monoclonal antibodyThen the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control. (1:500)

Supportive validation

Submitted by

LifeSpan BioSciences, Inc. (provider)

Main image

Experimental details

Equivalent amounts of cell lysates and LGALS3-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-LGALS3 monoclonal antibodyThen the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control. (1:500)