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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX113486 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113486, RRID:AB_11164060
- Product name
- Galectin 3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat, Canine
- Host
- Rabbit
Submitted references Ticagrelor attenuates myocardial ischaemia-reperfusion injury possibly through downregulating galectin-3 expression in the infarct area of rats.
Galectin-3 modulates the polarized surface delivery of β1-integrin in epithelial cells.
Liu X, Gu Y, Liu Y, Zhang M, Wang Y, Hu L
British journal of clinical pharmacology 2018 Jun;84(6):1180-1186
British journal of clinical pharmacology 2018 Jun;84(6):1180-1186
Galectin-3 modulates the polarized surface delivery of β1-integrin in epithelial cells.
Hönig E, Ringer K, Dewes J, von Mach T, Kamm N, Kreitzer G, Jacob R
Journal of cell science 2018 Jun 11;131(11)
Journal of cell science 2018 Jun 11;131(11)
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE GTX113486 diluted at 1:10000
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- GeneTex (provider)
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- Experimental details
- Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: LGALS3 transfected 293T lysates 12% SDS PAGE GTX113486 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Galectin3 antibody detects Galectin3 protein by western blot analysis.A. 50 £gg rat colon lysate/extract12 % SDS-PAGEGalectin3 antibody (GTX113486) dilution: 1:10000
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- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Galectin3 antibody detects Galectin3 protein at cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Galectin3 protein stained by Galectin3 antibody (GTX113486) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Galectin3(GTX113486) antibody at 1:500 dilution.
