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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- NB300-538 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-538, RRID:AB_10001365
- Product name
- Mouse Monoclonal Galectin-3 Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified. Galectin 3 (A3A12)
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Mouse
- Antigen sequence
The epitope for this antibody has b
een mapped to the first 58 amino ac
ids of Galectin 3.- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Hepatoprotective effects of the dual peroxisome proliferator-activated receptor alpha/delta agonist, GFT505, in rodent models of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis.
Modulation of functional properties of galectin-3 by monoclonal antibodies binding to the non-lectin domains.
Staels B, Rubenstrunk A, Noel B, Rigou G, Delataille P, Millatt LJ, Baron M, Lucas A, Tailleux A, Hum DW, Ratziu V, Cariou B, Hanf R
Hepatology (Baltimore, Md.) 2013 Dec;58(6):1941-52
Hepatology (Baltimore, Md.) 2013 Dec;58(6):1941-52
Modulation of functional properties of galectin-3 by monoclonal antibodies binding to the non-lectin domains.
Liu FT, Hsu DK, Zuberi RI, Hill PN, Shenhav A, Kuwabara I, Chen SS
Biochemistry 1996 May 14;35(19):6073-9
Biochemistry 1996 May 14;35(19):6073-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human ovarian carcinoma (right) compared with a negative control in the absence of primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control in the absence of primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control in the absence of primary antibody (left).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Galectin-3 Antibody (A3A12) [NB300-538] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HT-29 cells with Galectin-3 (A3A12) antibody NB300-538AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
