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- References [6]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF1154 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Galectin-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Galectin-3 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Galectin-3 is observed and less than 1% cross-reactivity with recombinant human Galectin-8 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P17931.5- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Human trophoblast requires galectin-3 for cell migration and invasion.
Intranuclear accumulation of galectin-3 is an independent prognostic factor for patients with distal cholangiocarcinoma.
Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury.
Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.
The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis.
Bojić-Trbojević Ž, Jovanović Krivokuća M, Vilotić A, Kolundžić N, Stefanoska I, Zetterberg F, Nilsson UJ, Leffler H, Vićovac L
Scientific reports 2019 Feb 14;9(1):2136
Scientific reports 2019 Feb 14;9(1):2136
Intranuclear accumulation of galectin-3 is an independent prognostic factor for patients with distal cholangiocarcinoma.
Shimura T, Kofunato Y, Okada R, Yashima R, Koyama Y, Araki K, Kuwano H, Takenoshita S
Oncology letters 2017 Jul;14(1):819-829
Oncology letters 2017 Jul;14(1):819-829
Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury.
Ryu KH, Kim SY, Kim YR, Woo SY, Sung SH, Kim HS, Jung SC, Jo I, Park JW
Experimental cell research 2014 Aug 1;326(1):143-54
Experimental cell research 2014 Aug 1;326(1):143-54
Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.
Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernández MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):242-54
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):242-54
The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
Thijssen VL, Hulsmans S, Griffioen AW
The American journal of pathology 2008 Feb;172(2):545-53
The American journal of pathology 2008 Feb;172(2):545-53
Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis.
Zhuo Y, Chammas R, Bellis SL
The Journal of biological chemistry 2008 Aug 8;283(32):22177-85
The Journal of biological chemistry 2008 Aug 8;283(32):22177-85
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Galectin-3 by Simple Western<SUP abp="265">TM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3 at approximately 37-38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Galectin-3 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Galectin-3 in Human Colon. Galectin-3 was detected in formalin fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
