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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-78096 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUCB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, HeLa, HepG2
- Concentration
- 1.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NUCB2 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with NUCB2 polyclonal antibody (Product # PA5-78096) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NUCB2 Polyclonal Antibody detects NUCB2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUCB2 Polyclonal Antibody (Product # PA5-78096) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NUCB2 Polyclonal Antibody detects NUCB2 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NUCB2 protein stained by NUCB2 Polyclonal Antibody (Product # PA5-78096) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NUCB2 Polyclonal Antibody detects NUCB2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NUCB2 Polyclonal Antibody (Product # PA5-78096) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
