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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-87383 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87383, RRID:AB_11014709
- Product name
- Rabbit Polyclonal Nesfatin-1/Nucleobindin-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Nesfatin-1/Nucleobindin-2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Effects of cannabinoid modulation on hypothalamic nesfatin-1 and insulin resistance.
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Kaya O, Yilmaz ME, Bayram S, Gunduz O, Kizilay G, Ozturk L
The Chinese journal of physiology 2019 Sep-Oct;62(5):182-187
The Chinese journal of physiology 2019 Sep-Oct;62(5):182-187
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51
Journal of proteomics 2012 Apr 3;75(7):2236-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Analysis in human cell line HL-60.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP1-87383] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
