HPA005468

antibody from Atlas Antibodies

Targeting: ASAH1 AC, ACDase, ASAH, FLJ21558, PHP32

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA005468

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA005468, RRID:AB_1845062

Product name

Anti-ASAH1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

ENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGC
VITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHP
FFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKP
VLNKLTVYTTLIDVTKGQF


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells

Cai K, Lucki N, Sewer M
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2014 April;1841(4):552-562

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Acid ceramidase (ASAH1) represses steroidogenic factor 1-dependent gene transcription in H295R human adrenocortical cells by binding to the receptor.

Lucki NC, Li D, Bandyopadhyay S, Wang E, Merrill AH, Sewer MB
Molecular and cellular biology 2012 Nov;32(21):4419-31

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Acid ceramidase (ASAH1) is a global regulator of steroidogenic capacity and adrenocortical gene expression.

Lucki NC, Bandyopadhyay S, Wang E, Merrill AH, Sewer MB
Molecular endocrinology (Baltimore, Md.) 2012 Feb;26(2):228-43

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Genistein stimulates MCF-7 breast cancer cell growth by inducing acid ceramidase (ASAH1) gene expression.

Lucki NC, Sewer MB
The Journal of biological chemistry 2011 Jun 3;286(22):19399-409

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The cAMP-responsive element binding protein (CREB) regulates the expression of acid ceramidase (ASAH1) in H295R human adrenocortical cells.

Lucki N, Sewer MB
Biochimica et biophysica acta 2009 Aug;1791(8):706-13

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines SK-MEL-30 and U-251MG using Anti-ASAH1 antibody. Corresponding ASAH1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human epididymis shows strong granular positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in a subset of renal tubules.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.

Sample type

HUMAN