NB300-318

antibody from Novus Biologicals

Targeting: SLC7A11 xCT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-318

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-318, RRID:AB_10000581

Product name

Rabbit Polyclonal xCT Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Dysregulation of Glutamate Transport Enhances Treg Function That Promotes VEGF Blockade Resistance in Glioblastoma.

Long Y, Tao H, Karachi A, Grippin AJ, Jin L, Chang YE, Zhang W, Dyson KA, Hou AY, Na M, Deleyrolle LP, Sayour EJ, Rahman M, Mitchell DA, Lin Z, Huang J
Cancer research 2020 Feb 1;80(3):499-509

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Endothelial-Mesenchymal Transition Drives Expression of CD44 Variant and xCT in Pulmonary Hypertension.

Isobe S, Kataoka M, Endo J, Moriyama H, Okazaki S, Tsuchihashi K, Katsumata Y, Yamamoto T, Shirakawa K, Yoshida N, Shimoda M, Chiba T, Masuko T, Hakamata Y, Kobayashi E, Saya H, Fukuda K, Sano M
American journal of respiratory cell and molecular biology 2019 Sep;61(3):367-379

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Influx rate of ¹⁸F-fluoroaminosuberic acid reflects cystine/glutamate antiporter expression in tumour xenografts.

Pitman KE, Alluri SR, Kristian A, Aarnes EK, Lyng H, Riss PJ, Malinen E
European journal of nuclear medicine and molecular imaging 2019 Sep;46(10):2190-2198

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Immunosuppressive Immature Myeloid Cell Generation Is Controlled by Glutamine Metabolism in Human Cancer.

Wu WC, Sun HW, Chen J, OuYang HY, Yu XJ, Chen HT, Shuang ZY, Shi M, Wang Z, Zheng L
Cancer immunology research 2019 Oct;7(10):1605-1618

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mTOR-dependent upregulation of xCT blocks melanin synthesis and promotes tumorigenesis.

Li C, Chen H, Lan Z, He S, Chen R, Wang F, Liu Z, Li K, Cheng L, Liu Y, Sun K, Wan X, Chen X, Peng H, Li L, Zhang Y, Jing Y, Huang M, Wang Y, Wang Y, Jiang J, Zha X, Chen L, Zhang H
Cell death and differentiation 2019 Oct;26(10):2015-2028

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xCT knockdown in human breast cancer cells delays onset of cancer-induced bone pain.

Ungard RG, Linher-Melville K, Nashed MG, Sharma M, Wen J, Singh G
Molecular pain 2019 Jan-Dec;15:1744806918822185

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Measurement of Tumor Antioxidant Capacity and Prediction of Chemotherapy Resistance in Preclinical Models of Ovarian Cancer by Positron Emission Tomography.

Greenwood HE, McCormick PN, Gendron T, Glaser M, Pereira R, Maddocks ODK, Sander K, Zhang T, Koglin N, Lythgoe MF, Årstad E, Hochhauser D, Witney TH
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Apr 15;25(8):2471-2482

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Altered glutamate clearance in ascorbate deficient mice increases seizure susceptibility and contributes to cognitive impairment in APP/PSEN1 mice.

Mi DJ, Dixit S, Warner TA, Kennard JA, Scharf DA, Kessler ES, Moore LM, Consoli DC, Bown CW, Eugene AJ, Kang JQ, Harrison FE
Neurobiology of aging 2018 Nov;71:241-254

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The effects of ceftriaxone on cue-primed reinstatement of cocaine-seeking in male and female rats: estrous cycle effects on behavior and protein expression in the nucleus accumbens.

Bechard AR, Hamor PU, Schwendt M, Knackstedt LA
Psychopharmacology 2018 Mar;235(3):837-848

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Functional effects of TrkA inhibition on system x_C^--mediated glutamate release and cancer-induced bone pain.

Miladinovic T, Ungard RG, Linher-Melville K, Popovic S, Singh G
Molecular pain 2018 Jan-Dec;14:1744806918776467

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[(18)F]FSPG-PET reveals increased cystine/glutamate antiporter (xc-) activity in a mouse model of multiple sclerosis.

Hoehne A, James ML, Alam IS, Ronald JA, Schneider B, D'Souza A, Witney TH, Andrews LE, Cropper HC, Behera D, Gowrishankar G, Ding Z, Wyss-Coray T, Chin FT, Biswal S, Gambhir SS
Journal of neuroinflammation 2018 Feb 22;15(1):55

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Characterization of the cystine/glutamate antiporter in cultured Bergmann glia cells.

Suárez-Pozos E, Martínez-Lozada Z, Méndez-Flores OG, Guillem AM, Hernández-Kelly LC, Castelán F, Olivares-Bañuelos TN, Chi-Castañeda D, Najimi M, Ortega A
Neurochemistry international 2017 Sep;108:52-59

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Activation of the NRF2 antioxidant program generates an imbalance in central carbon metabolism in cancer.

Sayin VI, LeBoeuf SE, Singh SX, Davidson SM, Biancur D, Guzelhan BS, Alvarez SW, Wu WL, Karakousi TR, Zavitsanou AM, Ubriaco J, Muir A, Karagiannis D, Morris PJ, Thomas CJ, Possemato R, Vander Heiden MG, Papagiannakopoulos T
eLife 2017 Oct 2;6

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Role of the Astroglial Glutamate Exchanger xCT in Ventral Hippocampus in Resilience to Stress.

Nasca C, Bigio B, Zelli D, de Angelis P, Lau T, Okamoto M, Soya H, Ni J, Brichta L, Greengard P, Neve RL, Lee FS, McEwen BS
Neuron 2017 Oct 11;96(2):402-413.e5

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Erythropoietin-induced cytoprotection in intestinal epithelial cells is linked to system Xc<sup/>.

Martin C, Patel M, Melendez-Ferro M, Sims B
Experimental cell research 2017 Mar 15;352(2):202-206

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Contrasting the Role of xCT and GLT-1 Upregulation in the Ability of Ceftriaxone to Attenuate the Cue-Induced Reinstatement of Cocaine Seeking and Normalize AMPA Receptor Subunit Expression.

LaCrosse AL, O'Donovan SM, Sepulveda-Orengo MT, McCullumsmith RE, Reissner KJ, Schwendt M, Knackstedt LA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Jun 14;37(24):5809-5821

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Absence of system x(c)(-) on immune cells invading the central nervous system alleviates experimental autoimmune encephalitis.

Merckx E, Albertini G, Paterka M, Jensen C, Albrecht P, Dietrich M, Van Liefferinge J, Bentea E, Verbruggen L, Demuyser T, Deneyer L, Lewerenz J, van Loo G, De Keyser J, Sato H, Maher P, Methner A, Massie A
Journal of neuroinflammation 2017 Jan 13;14(1):9

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CD44 variant isoform 9 emerges in response to injury and contributes to the regeneration of the gastric epithelium.

Bertaux-Skeirik N, Wunderlich M, Teal E, Chakrabarti J, Biesiada J, Mahe M, Sundaram N, Gabre J, Hawkins J, Jian G, Engevik AC, Yang L, Wang J, Goldenring JR, Qualls JE, Medvedovic M, Helmrath MA, Diwan T, Mulloy JC, Zavros Y
The Journal of pathology 2017 Aug;242(4):463-475

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Chronic Inhibition of STAT3/STAT5 in Treatment-Resistant Human Breast Cancer Cell Subtypes: Convergence on the ROS/SUMO Pathway and Its Effects on xCT Expression and System xc- Activity.

Linher-Melville K, Nashed MG, Ungard RG, Haftchenary S, Rosa DA, Gunning PT, Singh G
PloS one 2016;11(8):e0161202

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The cystine/glutamate antiporter system xc- drives breast tumor cell glutamate release and cancer-induced bone pain.

Slosky LM, BassiriRad NM, Symons AM, Thompson M, Doyle T, Forte BL, Staatz WD, Bui L, Neumann WL, Mantyh PW, Salvemini D, Largent-Milnes TM, Vanderah TW
Pain 2016 Nov;157(11):2605-2616

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Alcohol consumption increases basal extracellular glutamate in the nucleus accumbens core of Sprague-Dawley rats without increasing spontaneous glutamate release.

Pati D, Kelly K, Stennett B, Frazier CJ, Knackstedt LA
The European journal of neuroscience 2016 Jul;44(2):1896-905

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Utility of [¹⁸F]FSPG PET to Image Hepatocellular Carcinoma: First Clinical Evaluation in a US Population.

Kavanaugh G, Williams J, Morris AS, Nickels ML, Walker R, Koglin N, Stephens AW, Washington MK, Geevarghese SK, Liu Q, Ayers D, Shyr Y, Manning HC
Molecular imaging and biology 2016 Dec;18(6):924-934

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Increased Expression of System xc- in Glioblastoma Confers an Altered Metabolic State and Temozolomide Resistance.

Polewski MD, Reveron-Thornton RF, Cherryholmes GA, Marinov GK, Cassady K, Aboody KS
Molecular cancer research : MCR 2016 Dec;14(12):1229-1242

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Cystine/glutamate antiporter blockage induces myelin degeneration.

Soria FN, Zabala A, Pampliega O, Palomino A, Miguelez C, Ugedo L, Sato H, Matute C, Domercq M
Glia 2016 Aug;64(8):1381-95

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Comparative analysis of antibodies to xCT (Slc7a11): Forewarned is forearmed.

Van Liefferinge J, Bentea E, Demuyser T, Albertini G, Follin-Arbelet V, Holmseth S, Merckx E, Sato H, Aerts JL, Smolders I, Arckens L, Danbolt NC, Massie A
The Journal of comparative neurology 2016 Apr 1;524(5):1015-32

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Effects of (R)-(-)-5-methyl-1-nicotinoyl-2-pyrazoline on glutamate transporter 1 and cysteine/glutamate exchanger as well as ethanol drinking behavior in male, alcohol-preferring rats.

Aal-Aaboda M, Alhaddad H, Osowik F, Nauli SM, Sari Y
Journal of neuroscience research 2015 Jun;93(6):930-7

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Effects of ceftriaxone on GLT1 isoforms, xCT and associated signaling pathways in P rats exposed to ethanol.

Rao PS, Saternos H, Goodwani S, Sari Y
Psychopharmacology 2015 Jul;232(13):2333-42

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Stress-induced inhibition of nonsense-mediated RNA decay regulates intracellular cystine transport and intracellular glutathione through regulation of the cystine/glutamate exchanger SLC7A11.

Martin L, Gardner LB
Oncogene 2015 Aug 6;34(32):4211-8

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Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype.

Lu H, Samanta D, Xiang L, Zhang H, Hu H, Chen I, Bullen JW, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 18;112(33):E4600-9

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Lack of effect of Theiler's murine encephalomyelitis virus infection on system xc⁻.

Merckx E, Demuyser T, Bentea E, Van Liefferinge J, Albertini G, Deneyer L, Michiels T, Massie A
Neuroscience letters 2015 Apr 23;593:124-8

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Kaposi's Sarcoma-Associated Herpesvirus Subversion of the Anti-Inflammatory Response in Human Skin Cells Reveals Correlates of Latency and Disease Pathogenesis.

Fontana JM, Mygatt JG, Conant KL, Parsons CH, Kaleeba JA
Journal of skin cancer 2014;2014:246076

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Image-based RNA interference screening reveals an individual dependence of acute lymphoblastic leukemia on stromal cysteine support.

Boutter J, Huang Y, Marovca B, Vonderheit A, Grotzer MA, Eckert C, Cario G, Wollscheid B, Horvath P, Bornhauser BC, Bourquin JP
Oncotarget 2014 Nov 30;5(22):11501-12

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IGF-I regulates redox status in breast cancer cells by activating the amino acid transport molecule xC-.

Yang Y, Yee D
Cancer research 2014 Apr 15;74(8):2295-305

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Reduced expression of miRNA-27a modulates cisplatin resistance in bladder cancer by targeting the cystine/glutamate exchanger SLC7A11.

Drayton RM, Dudziec E, Peter S, Bertz S, Hartmann A, Bryant HE, Catto JW
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Apr 1;20(7):1990-2000

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Early intraarticular injection of hyaluronic acid attenuates osteoarthritis progression in anterior cruciate ligament-transected rats.

Tsai WY, Wu JL, Liu CC, Cherng CH, Tsai RY, Jean YH, Wong CS
Connective tissue research 2013;54(1):49-54

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Glutamine sensitivity analysis identifies the xCT antiporter as a common triple-negative breast tumor therapeutic target.

Timmerman LA, Holton T, Yuneva M, Louie RJ, Padró M, Daemen A, Hu M, Chan DA, Ethier SP, van 't Veer LJ, Polyak K, McCormick F, Gray JW
Cancer cell 2013 Oct 14;24(4):450-65

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Targeting aberrant glutathione metabolism to eradicate human acute myelogenous leukemia cells.

Pei S, Minhajuddin M, Callahan KP, Balys M, Ashton JM, Neering SJ, Lagadinou ED, Corbett C, Ye H, Liesveld JL, O'Dwyer KM, Li Z, Shi L, Greninger P, Settleman J, Benes C, Hagen FK, Munger J, Crooks PA, Becker MW, Jordan CT
The Journal of biological chemistry 2013 Nov 22;288(47):33542-58

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The pharmacologic inhibition of the xc- antioxidant system improves the antitumor efficacy of COX inhibitors in the in vivo model of 3-MCA tumorigenesis.

Balza E, Castellani P, Delfino L, Truini M, Rubartelli A
Carcinogenesis 2013 Mar;34(3):620-6

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Inhibition of nonsense-mediated RNA decay activates autophagy.

Wengrod J, Martin L, Wang D, Frischmeyer-Guerrerio P, Dietz HC, Gardner LB
Molecular and cellular biology 2013 Jun;33(11):2128-35

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(4S)-4-(3-18F-fluoropropyl)-L-glutamate for imaging of xC transporter activity in hepatocellular carcinoma using PET: preclinical and exploratory clinical studies.

Baek S, Mueller A, Lim YS, Lee HC, Lee YJ, Gong G, Kim JS, Ryu JS, Oh SJ, Lee SJ, Bacher-Stier C, Fels L, Koglin N, Schatz CA, Dinkelborg LM, Moon DH
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2013 Jan;54(1):117-23

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Activated human CD4+ T cells express transporters for both cysteine and cystine.

Levring TB, Hansen AK, Nielsen BL, Kongsbak M, von Essen MR, Woetmann A, Odum N, Bonefeld CM, Geisler C
Scientific reports 2012;2:266

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Exploratory clinical trial of (4S)-4-(3-[18F]fluoropropyl)-L-glutamate for imaging xC- transporter using positron emission tomography in patients with non-small cell lung or breast cancer.

Baek S, Choi CM, Ahn SH, Lee JW, Gong G, Ryu JS, Oh SJ, Bacher-Stier C, Fels L, Koglin N, Hultsch C, Schatz CA, Dinkelborg LM, Mittra ES, Gambhir SS, Moon DH
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Oct 1;18(19):5427-37

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Extracellular human immunodeficiency virus type 1 viral protein R causes reductions in astrocytic ATP and glutathione levels compromising the antioxidant reservoir.

Ferrucci A, Nonnemacher MR, Cohen EA, Wigdahl B
Virus research 2012 Aug;167(2):358-69

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Astrocytic redox remodeling by amyloid beta peptide.

Garg SK, Vitvitsky V, Albin R, Banerjee R
Antioxidants & redox signaling 2011 Jun 15;14(12):2385-97

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Role of the Nrf2-ARE pathway in acrylamide neurotoxicity.

Zhang L, Gavin T, Barber DS, LoPachin RM
Toxicology letters 2011 Aug 10;205(1):1-7

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Glutamate, excitatory amino acid transporters, Xc- antiporter, glutamine synthetase, and gamma-glutamyltranspeptidase in human corneal epithelium.

Langford MP, Redmond P, Chanis R, Misra RP, Redens TB
Current eye research 2010 Mar;35(3):202-11

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Burn injury induces the expression of cystine/glutamate transporter (x(c)(-)) in mouse T cells.

D'Elia M, Patenaude J, Dupras C, Bernier J
Immunology letters 2009 Aug 15;125(2):137-44

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Cystine-glutamate transporter SLC7A11 mediates resistance to geldanamycin but not to 17-(allylamino)-17-demethoxygeldanamycin.

Liu R, Blower PE, Pham AN, Fang J, Dai Z, Wise C, Green B, Teitel CH, Ning B, Ling W, Lyn-Cook BD, Kadlubar FF, Sadée W, Huang Y
Molecular pharmacology 2007 Dec;72(6):1637-46

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: xCT Antibody [NB300-318] - Total protein from Human HeLa cells treated with and without 0.1 mM Diethyl Maleate for 24 hours was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-xCT in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the increase in xCT expression with treatment.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: xCT Antibody [NB300-318] - Lane view shows a specific band for xCT in Hep G2 cell lysate using 25 ug/ml antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 63. Image from an internal validation.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: xCT Antibody [NB300-318] - Simple Western lane view shows a specific band for xCT using NB300-318 at 25 ug/mL in HepG2 cell lysates and antibody at 25 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 63 kDa. Image reported by internal validation.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: xCT Antibody [NB300-318] - Simple Western lane view shows a specific band for xCT using NB300-318 at 25 ug/ml in HeLa and HeLa + DEM cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: xCT Antibody [NB300-318] - Western blot analysis of protein isolated from subcutaneous tumors derived from in vivo growth of the clones relative to WT-derived tumors revealed that xCT levels remained low, phospho-STAT5 (p-STAT5) levels remained high, and phospho-STAT3 (p-STAT3) levels remained unchanged in the absence of SH-4-54. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0161202), licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: xCT Antibody [NB300-318] - xCT staining in the absorptive epithelia of small intestinal villi detected using NB300-318.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa with xCT Antibody NB300-318 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (F0111, R&D Systems).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: xCT Antibody [NB300-318] - An intracellular stain was performed on HeLa cells with xCT Antibody NB300-318AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.