MAB1187-100

antibody from R&D Systems

Targeting: TREM1 CD354, TREM-1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1187-100

Provider

R&D Systems

Product name

Mouse TREM-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse TREM-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse TREM-2B, recombinant human (rh) TREM-1, or rhTREM-2 is observed.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

Q9JKE2


Isotype

IgG

Antibody clone number

174031

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

TREM-1 regulates neutrophil chemotaxis by promoting NOX-dependent superoxide production.

Baruah S, Murthy S, Keck K, Galvan I, Prichard A, Allen LH, Farrelly M, Klesney-Tait J
Journal of leukocyte biology 2019 Jun;105(6):1195-1207

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HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1.

Yuan Z, Fan X, Staitieh B, Bedi C, Spearman P, Guidot DM, Sadikot RT
Scientific reports 2017 Feb 9;7:42028

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TREM-2 promotes macrophage survival and lung disease after respiratory viral infection.

Wu K, Byers DE, Jin X, Agapov E, Alexander-Brett J, Patel AC, Cella M, Gilfilan S, Colonna M, Kober DL, Brett TJ, Holtzman MJ
The Journal of experimental medicine 2015 May 4;212(5):681-97

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TREM-1 deficiency can attenuate disease severity without affecting pathogen clearance.

Weber B, Schuster S, Zysset D, Rihs S, Dickgreber N, Schürch C, Riether C, Siegrist M, Schneider C, Pawelski H, Gurzeler U, Ziltener P, Genitsch V, Tacchini-Cottier F, Ochsenbein A, Hofstetter W, Kopf M, Kaufmann T, Oxenius A, Reith W, Saurer L, Mueller C
PLoS pathogens 2014 Jan;10(1):e1003900

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Comparison of gene expression profiles between human and mouse monocyte subsets.

Ingersoll MA, Spanbroek R, Lottaz C, Gautier EL, Frankenberger M, Hoffmann R, Lang R, Haniffa M, Collin M, Tacke F, Habenicht AJ, Ziegler-Heitbrock L, Randolph GJ
Blood 2010 Jan 21;115(3):e10-9

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TREM-1 activation alters the dynamics of pulmonary IRAK-M expression in vivo and improves host defense during pneumococcal pneumonia.

Lagler H, Sharif O, Haslinger I, Matt U, Stich K, Furtner T, Doninger B, Schmid K, Gattringer R, de Vos AF, Knapp S
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 1;183(3):2027-36

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

TREM-1 in Mouse Splenocytes. TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse TREM-1 Monoclonal Antibody (Catalog # MAB1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of TREM-1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM-1 Monoclonal Antibody (Catalog # MAB1187) or (B) Rat IgG_2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).