15023-1-AP

antibody from Proteintech Group

Targeting: CPSF4 CPSF30, NAR

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15023-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15023-1-AP, RRID:AB_2084544

Product name

CPSF4 antibody

Antibody type

Polyclonal

Description

KD/KO validated CPSF4 antibody (Cat. #15023-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT.

Yang Q, Fan W, Zheng Z, Lin S, Liu C, Wang R, Li W, Zuo Y, Sun Y, Hu S, Chen M, Guo P, Pan J, Tian C, Zhu T, Diao C, Hao J, Yu W, Li L, Li Y, Guo W, Deng W, Wu X
Biochimica et biophysica acta. Molecular cell research 2019 Oct;1866(10):1533-1543

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The polyadenylation inhibitor cordycepin reduces pain, inflammation and joint pathology in rodent models of osteoarthritis.

Ashraf S, Radhi M, Gowler P, Burston JJ, Gandhi RD, Thorn GJ, Piccinini AM, Walsh DA, Chapman V, de Moor CH
Scientific reports 2019 Mar 18;9(1):4696

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MiR-4458 inhibits breast cancer cell growth, migration, and invasiveness by targeting CPSF4.

Wu J, Miao J, Ding Y, Zhang Y, Huang X, Zhou X, Tang R
Biochemistry and cell biology = Biochimie et biologie cellulaire 2019 Dec;97(6):722-730

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Cleavage and polyadenylation specific factor 4 targets NF-κB/cyclooxygenase-2 signaling to promote lung cancer growth and progression.

Yi C, Wang Y, Zhang C, Xuan Y, Zhao S, Liu T, Li W, Liao Y, Feng X, Hao J, Gao Y, Yu W, Chen Y, Zhang C, Guo W, Tang B, Deng W
Cancer letters 2016 Oct 10;381(1):1-13

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CREB-binding protein regulates lung cancer growth by targeting MAPK and CPSF4 signaling pathway.

Tang Z, Yu W, Zhang C, Zhao S, Yu Z, Xiao X, Tang R, Xuan Y, Yang W, Hao J, Xu T, Zhang Q, Huang W, Deng W, Guo W
Molecular oncology 2016 Feb;10(2):317-29

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CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas.

Chen W, Qin L, Wang S, Li M, Shi D, Tian Y, Wang J, Fu L, Li Z, Guo W, Yu W, Yuan Y, Kang T, Huang W, Deng W
Molecular oncology 2014 May;8(3):704-16

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Upregulation of cleavage and polyadenylation specific factor 4 in lung adenocarcinoma and its critical role for cancer cell survival and proliferation.

Chen W, Guo W, Li M, Shi D, Tian Y, Li Z, Wang J, Fu L, Xiao X, Liu QQ, Wang S, Huang W, Deng W
PloS one 2013;8(12):e82728

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 15023-1-AP(CPSF4 antibody) at dilution of 1:300

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CPSF4 antibody 15023-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-CPSF4 (IP:15023-1-AP, 3ug; Detection:15023-1-AP 1:400) with HeLa cells lysate 1000ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human endometrial cancer using 15023-1-AP(CPSF4 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue