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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB2926-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse BMP-10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BMP-10 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BMP-1.1, 2, 3, 3b, 4, 5, 6, 7, 9, 15, or the pro region of rhBMP-10 is observed. In direct ELISAs, approximately 90% cross-reactivity with recombinant mouse BMP-10 is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O95393- Isotype
- IgG
- Antibody clone number
- 462732
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A heterodimer formed by bone morphogenetic protein 9 (BMP9) and BMP10 provides most BMP biological activity in plasma.
A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10.
BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus.
BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
Systematic analysis of gene expression differences between left and right atria in different mouse strains and in human atrial tissue.
Tillet E, Ouarné M, Desroches-Castan A, Mallet C, Subileau M, Didier R, Lioutsko A, Belthier G, Feige JJ, Bailly S
The Journal of biological chemistry 2018 Jul 13;293(28):10963-10974
The Journal of biological chemistry 2018 Jul 13;293(28):10963-10974
A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10.
Ruiz S, Zhao H, Chandakkar P, Chatterjee PK, Papoin J, Blanc L, Metz CN, Campagne F, Marambaud P
Scientific reports 2016 Nov 22;5:37366
Scientific reports 2016 Nov 22;5:37366
BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus.
Levet S, Ouarné M, Ciais D, Coutton C, Subileau M, Mallet C, Ricard N, Bidart M, Debillon T, Faravelli F, Rooryck C, Feige JJ, Tillet E, Bailly S
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun 23;112(25):E3207-15
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun 23;112(25):E3207-15
BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
Ricard N, Ciais D, Levet S, Subileau M, Mallet C, Zimmers TA, Lee SJ, Bidart M, Feige JJ, Bailly S
Blood 2012 Jun 21;119(25):6162-71
Blood 2012 Jun 21;119(25):6162-71
Systematic analysis of gene expression differences between left and right atria in different mouse strains and in human atrial tissue.
Kahr PC, Piccini I, Fabritz L, Greber B, Schöler H, Scheld HH, Hoffmeier A, Brown NA, Kirchhof P
PloS one 2011;6(10):e26389
PloS one 2011;6(10):e26389
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Alkaline Phosphatase Production Induced by BMP-10 and Neutralization by Human BMP-10 Antibody. Recombinant Human BMP-10 (Catalog # 2926-BP) induces alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP-10 Monoclonal Antibody (Catalog # MAB2926). The ND50 is typically 0.15-0.9 µg/mL.
