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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- MA1-41059 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TEM8 Monoclonal Antibody (200C1339 (SB20))
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: TEM8 transfected cell lysate.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 200C1339 (SB20)
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of TEM8 in TEM8 transfected cell lysate using a Tumor Endothelial Marker 8 monoclonal antibody (Product # MA1-41059) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TEM8 in 35 µg/lane human heart lysates. Samples were incubated in TEM8 monoclonal antibody (Product # MA1-41059 using a dilution of 0.03 µg/mL. Band observed at 65 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TEM8 in transfected cell lysate. Sample was incubated in TEM8 monoclonal antibody (Product # MA1-41059).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TEM8 in human liver cancer section. Samples were incubated in TEM8 monoclonal antibody (Product # MA1-41059) using a dilution of 5 µg/mL. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TEM8 in Human colon. Samples were incubated in TEM8 monoclonal antibody (Product # MA1-41059) using a dilution of 2.5 µg/mL. DAB chromogen detection method (10X magnification).
