LS-C396229

antibody from LSBio

Targeting: CLGN

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C396229

Provider

LSBio

Product name

CLGN / Calmegin Antibody LS-C396229

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot. All lanes CLGN antibody at 7 ug/ml. Lane 1: Mouse brain tissue. Lane 2: Mouse lung tissue. Lane 3: Jurkat whole cell lysate. Lane 4: HepG-2 whole cell lysate. Lane 5: PC-3 whole cell lysate. Lane 6: HeLa whole cell lysate. Lane 7: HL60 whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 70 kDa. Observed band size: 80 kDa.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: CLGN antibody at 7µg/ml Lane 1: Mouse brain tissue Lane 2: Mouse lung tissue Lane 3: Jurkat whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: PC-3 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 47 kDa Observed band size: 80 kDa

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue using antibody at dilution of 1:100.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human endometrial cancer using antibody at dilution of 1:100.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue using antibody at dilution of 1:100.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human endometrial cancer using antibody at dilution of 1:100.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue using antibody at dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human endometrial cancer using antibody at dilution of 1:100.