Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [1]
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Validation data
Reference
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- Product number
- MAB6158 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal UCP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse UCP1 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references BMP4 gene therapy enhances insulin sensitivity but not adipose tissue browning in obese mice.
Differentiating SGBS adipocytes respond to PPARγ stimulation, irisin and BMP7 by functional browning and beige characteristics.
Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes.
Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity.
A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome.
IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk.
Both brown adipose tissue and skeletal muscle thermogenesis processes are activated during mild to severe cold adaptation in mice.
Direct conversion of human fibroblasts to brown adipocytes by small chemical compounds.
White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
Hoffmann JM, Grünberg JR, Hammarstedt A, Kroon T, Greiner TU, Maurer S, Elias I, Palsdottir V, Bosch F, Boucher J, Hedjazifar S, Smith U
Molecular metabolism 2020 Feb;32:15-26
Molecular metabolism 2020 Feb;32:15-26
Differentiating SGBS adipocytes respond to PPARγ stimulation, irisin and BMP7 by functional browning and beige characteristics.
Klusóczki Á, Veréb Z, Vámos A, Fischer-Posovszky P, Wabitsch M, Bacso Z, Fésüs L, Kristóf E
Scientific reports 2019 Apr 9;9(1):5823
Scientific reports 2019 Apr 9;9(1):5823
Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes.
Harms MJ, Li Q, Lee S, Zhang C, Kull B, Hallen S, Thorell A, Alexandersson I, Hagberg CE, Peng XR, Mardinoglu A, Spalding KL, Boucher J
Cell reports 2019 Apr 2;27(1):213-225.e5
Cell reports 2019 Apr 2;27(1):213-225.e5
Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity.
Hagberg CE, Li Q, Kutschke M, Bhowmick D, Kiss E, Shabalina IG, Harms MJ, Shilkova O, Kozina V, Nedergaard J, Boucher J, Thorell A, Spalding KL
Cell reports 2018 Sep 4;24(10):2746-2756.e5
Cell reports 2018 Sep 4;24(10):2746-2756.e5
A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome.
Su S, Guntur AR, Nguyen DC, Fakory SS, Doucette CC, Leech C, Lotana H, Kelley M, Kohli J, Martino J, Sims-Lucas S, Liaw L, Vary C, Rosen CJ, Brown AC
Cell reports 2018 Dec 11;25(11):3215-3228.e9
Cell reports 2018 Dec 11;25(11):3215-3228.e9
IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk.
Zou Y, Lu P, Shi J, Liu W, Yang M, Zhao S, Chen N, Chen M, Sun Y, Gao A, Chen Q, Zhang Z, Ma Q, Ning T, Ying X, Jin J, Deng X, Shen B, Zhang Y, Yuan B, Kauderer S, Liu S, Hong J, Liu R, Ning G, Wang W, Gu W, Wang J
EBioMedicine 2017 Oct;24:64-75
EBioMedicine 2017 Oct;24:64-75
Both brown adipose tissue and skeletal muscle thermogenesis processes are activated during mild to severe cold adaptation in mice.
Bal NC, Singh S, Reis FCG, Maurya SK, Pani S, Rowland LA, Periasamy M
The Journal of biological chemistry 2017 Oct 6;292(40):16616-16625
The Journal of biological chemistry 2017 Oct 6;292(40):16616-16625
Direct conversion of human fibroblasts to brown adipocytes by small chemical compounds.
Takeda Y, Harada Y, Yoshikawa T, Dai P
Scientific reports 2017 Jun 27;7(1):4304
Scientific reports 2017 Jun 27;7(1):4304
White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
Moisan A, Lee YK, Zhang JD, Hudak CS, Meyer CA, Prummer M, Zoffmann S, Truong HH, Ebeling M, Kiialainen A, Gérard R, Xia F, Schinzel RT, Amrein KE, Cowan CA
Nature cell biology 2015 Jan;17(1):57-67
Nature cell biology 2015 Jan;17(1):57-67
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse UCP1 by Simple WesternTM. Detection of Mouse UCP1 by Simple WesternTM.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse UCP1 by Western Blot. Detection of Mouse UCP1 by Western Blot.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human UCP1 by Western Blot. Detection of Human UCP1 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of UCP1 in 3T3-L1 Mouse Cell Line by Flow Cytometry. Detection of UCP1 in 3T3-L1 Mouse Cell Line by Flow Cytometry.