MAB6158

antibody from R&D Systems

Targeting: UCP1 SLC25A7, UCP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6158

Provider

R&D Systems

Product name

Human/Mouse UCP1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse UCP1 in Western blots.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P25874


Isotype

IgG

Antibody clone number

536435

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Differentiating SGBS adipocytes respond to PPARγ stimulation, irisin and BMP7 by functional browning and beige characteristics.

Klusóczki Á, Veréb Z, Vámos A, Fischer-Posovszky P, Wabitsch M, Bacso Z, Fésüs L, Kristóf E
Scientific reports 2019 Apr 9;9(1):5823

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Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes.

Harms MJ, Li Q, Lee S, Zhang C, Kull B, Hallen S, Thorell A, Alexandersson I, Hagberg CE, Peng XR, Mardinoglu A, Spalding KL, Boucher J
Cell reports 2019 Apr 2;27(1):213-225.e5

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Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity.

Hagberg CE, Li Q, Kutschke M, Bhowmick D, Kiss E, Shabalina IG, Harms MJ, Shilkova O, Kozina V, Nedergaard J, Boucher J, Thorell A, Spalding KL
Cell reports 2018 Sep 4;24(10):2746-2756.e5

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A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome.

Su S, Guntur AR, Nguyen DC, Fakory SS, Doucette CC, Leech C, Lotana H, Kelley M, Kohli J, Martino J, Sims-Lucas S, Liaw L, Vary C, Rosen CJ, Brown AC
Cell reports 2018 Dec 11;25(11):3215-3228.e9

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IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk.

Zou Y, Lu P, Shi J, Liu W, Yang M, Zhao S, Chen N, Chen M, Sun Y, Gao A, Chen Q, Zhang Z, Ma Q, Ning T, Ying X, Jin J, Deng X, Shen B, Zhang Y, Yuan B, Kauderer S, Liu S, Hong J, Liu R, Ning G, Wang W, Gu W, Wang J
EBioMedicine 2017 Oct;24:64-75

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Both brown adipose tissue and skeletal muscle thermogenesis processes are activated during mild to severe cold adaptation in mice.

Bal NC, Singh S, Reis FCG, Maurya SK, Pani S, Rowland LA, Periasamy M
The Journal of biological chemistry 2017 Oct 6;292(40):16616-16625

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Direct conversion of human fibroblasts to brown adipocytes by small chemical compounds.

Takeda Y, Harada Y, Yoshikawa T, Dai P
Scientific reports 2017 Jun 27;7(1):4304

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White-to-brown metabolic conversion of human adipocytes by JAK inhibition.

Moisan A, Lee YK, Zhang JD, Hudak CS, Meyer CA, Prummer M, Zoffmann S, Truong HH, Ebeling M, Kiialainen A, Gérard R, Xia F, Schinzel RT, Amrein KE, Cowan CA
Nature cell biology 2015 Jan;17(1):57-67

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse UCP1 by Western Blot. Western blot shows lysates of mouse brown adipose tissue and mouse adipose tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCP1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human UCP1 by Western Blot. Western blot shows recombinant human UCP1, recombinant human UCP2, recombinant human UCP3, and recombinant human UCP4 (5 ng/lane). PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse UCP1 by Simple Western^TM. Simple Western lane view shows lysates of mouse adipose tissue and mouse brown adipose tissue, loaded at 0.5 mg/mL. A specific band was detected for UCP1 at approximately 37 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

UCP1 in Human Mesenchymal Stem Cells. UCP1 was detected in immersion fixed human mesenchymal stem cells undifferentiated (lower panel) or differentiated into adipocytes (upper panel) using Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of UCP1 in 3T3-L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.