MA5-17064

antibody from Invitrogen Antibodies

Targeting: CYP3A4 CYP3A3

Provider product page for MA5-17064

Western blot

Antibody data

Antibody Data
Antigen structure
References [6]
Comments [0]
Validations
Western blot [4]
Immunocytochemistry [1]
Immunohistochemistry [2]
Flow cytometry [1]
Other assay [1]

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Product number: MA5-17064 - Provider product page
Provider: Invitrogen Antibodies
Product name: CYP3A4 Monoclonal Antibody (3H8)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-17064 targets CYP3A4 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.
Antibody clone number: 3H8
Concentration: 1 mg/mL

CYP3A4 protein structure - MA5-17064 shown in red.

Submitted references Commiphora myrrha (Nees) Engl. resin extracts induce phase-I cytochrome P450 2C8, 2C9, 2C19, and 3A4 isoenzyme expressions in human hepatocellular carcinoma (HepG2) cells.

Alehaideb Z, Alatar G, Nehdi A, Albaz A, Al-Eidi H, Almutairi M, Hawsa E, Alshuail N, Matou-Nasri S
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society 2021 May;29(5):361-368

Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors.

Borys BS, Dang T, So T, Rohani L, Revay T, Walsh T, Thompson M, Argiropoulos B, Rancourt DE, Jung S, Hashimura Y, Lee B, Kallos MS
Stem cell research & therapy 2021 Jan 13;12(1):55

Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells.

Rohani L, Borys BS, Razian G, Naghsh P, Liu S, Johnson AA, Machiraju P, Holland H, Lewis IA, Groves RA, Toms D, Gordon PMK, Li JW, So T, Dang T, Kallos MS, Rancourt DE
Communications biology 2020 Sep 7;3(1):492

iTAG-RNA Isolates Cell-Specific Transcriptional Responses to Environmental Stimuli and Identifies an RNA-Based Endocrine Axis.

Darr J, Tomar A, Lassi M, Gerlini R, Berti L, Hering A, Scheid F, Hrabě de Angelis M, Witting M, Teperino R
Cell reports 2020 Mar 3;30(9):3183-3194.e4

Improving Drug Discovery by Nucleic Acid Delivery in Engineered Human Microlivers.

Mancio-Silva L, Fleming HE, Miller AB, Milstein S, Liebow A, Haslett P, Sepp-Lorenzino L, Bhatia SN
Cell metabolism 2019 Mar 5;29(3):727-735.e3

An in vitro system for measuring genotoxicity mediated by human CYP3A4 in Saccharomyces cerevisiae.

Fasullo M, Freedland J, St John N, Cera C, Egner P, Hartog M, Ding X
Environmental and molecular mutagenesis 2017 May;58(4):217-227

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CYP3A4 using a CYP3A4 monoclonal antibody (Product # MA5-17064) against a human CYP3A4 (AA: 243-430) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CYP3A4 using CYP3A4 monoclonal antibody (Product # MA5-17064) in HEK293 (1) and CYP3A4 (AA: 243-430) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CYP3A4 using a CYP3A4 monoclonal antibody (Product # MA5-17064) against a human CYP3A4 (AA: 243-430) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CYP3A4 using CYP3A4 monoclonal antibody (Product # MA5-17064) in HEK293 (1) and CYP3A4 (AA: 243-430) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of HepG2 cells using CYP3A4 monoclonal antibody (Product # MA5-17064) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CYP3A4 monoclonal antibody (Product # MA5-17064) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human brain tissues using CYP3A4 monoclonal antibody (Product # MA5-17064) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of HepG2 cells using CYP3A4 monoclonal antibody (Product # MA5-17064) (green) and negative control (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Engineering Liver Metabolism in MPCCs (A) CYP3A4 mRNA levels at day 3 post-transfection. Values (mean +- SEM, n = 2) were normalized to untreated MPCCs. p < 0.001. (B) Representative images of hepatocyte islands in MPCCs probed with CYP3A4 antibodies (green) on day 4 post-transfection. Hepatocyte and fibroblast nuclei are shown in blue. The white dotted line delineates the island border of hepatocytes. Scale bar, 50 mum. (C and D) Schematic reaction of the luminescence-based CYP3A4 activity assay (C) and relative CYP3A4 activity measured in MPCCs (D). Values were normalized to the corresponding untreated cells. Presented data are mean +- SEM (n = 3). p < 0.0001. (E) Relative human albumin levels secreted in MPCCs, as determined by ELISA. Values (mean +- SEM, n = 2) were normalized to the corresponding untreated condition. See Figure S1 A for urea levels. (F) CYP3A4 and CYP2D6 mRNA levels at day 3 post-transfection with CYP3A4 and CYP2D6 siRNA duplexes, alone or in combination. Graph bars represent mean +- SEM (n = 3). p < 0.01. (G) Relative CYP3A4 activity in the same conditions as (F), using proluciferin. Graph bars represent mean +- SEM (n = 2). **p < 0.01. *p < 0.05. See Figure S1 B for CYP3A4 activity measured with testosterone substrate.