PA5-72317

antibody from Invitrogen Antibodies

Targeting: SLC29A2 DER12, ENT2, HNP36

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-72317

Provider

Invitrogen Antibodies

Product name

ENT2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.48 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC29A2 in the following samples: Lane 1: human skeletal muscle lysate, Lane 2: HeLa whole cell lysate. 20 µg of protein per lane was used. Samples were first blocked with 5% NFDM/TBST, incubated with SLC29A2 polyclonal antibody (Product # PA5-72317) using a dilution of 1:2000 followed by a goat anti-rabbit IgG H&L (HRP) secondary antibody with a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC29A2 in the following samples: Lane 1: human skeletal muscle lysate, Lane 2: HeLa whole cell lysate. 20 µg of protein per lane was used. Samples were first blocked with 5% NFDM/TBST, incubated with SLC29A2 polyclonal antibody (Product # PA5-72317) using a dilution of 1:2000 followed by a goat anti-rabbit IgG H&L (HRP) secondary antibody with a dilution of 1:10,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SLC29A2 in paraffin-embedded human liver tissue. Samples were fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature. Antigen retrieval was performed by heat mediation with a citrate buffer (pH6). Samples were then incubated with SLC29A2 polyclonal antibody (Product # PA5-72317) using a dilution of 1:25 for 1 hours at 37°C. An undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Overlay histogram showing HepG2 cells stained with PA5-72317 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (PA5-72317, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of SLC29A2 in HepG2 cells (green). The cells were fixed with 2% paraformaldhyde (10 min) and permeabilized with 90% methanol (10 min). The sample was then incubated with 2% BSA followed by a SLC29A2 polyclonal antibody (Product # PA5-72317) using a dilution of 1:25 for 60 min at 37°C. The secondary antibody used was with a dilution of 1:200 for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 µg/1x10^6 cells) used under the same conditions with an acquisition of >10, 000 events.