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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005093-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005093-M01, RRID:AB_2160622
- Product name
- Mouse Monoclonal PCBP1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PCBP1 (1G2)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 50 ug
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references TGFβ promotes breast cancer stem cell self-renewal through an ILEI/LIFR signaling axis.
A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.
PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27.
ILEI drives epithelial to mesenchymal transition and metastatic progression in the lung cancer cell line A549.
Establishment of a TGFβ-induced post-transcriptional EMT gene signature.
Woosley AN, Dalton AC, Hussey GS, Howley BV, Mohanty BK, Grelet S, Dincman T, Bloos S, Olsen SK, Howe PH
Oncogene 2019 May;38(20):3794-3811
Oncogene 2019 May;38(20):3794-3811
A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.
Howley BV, Link LA, Grelet S, El-Sabban M, Howe PH
Oncogene 2018 Mar;37(10):1308-1325
Oncogene 2018 Mar;37(10):1308-1325
PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27.
Link LA, Howley BV, Hussey GS, Howe PH
Molecular cancer research : MCR 2016 Jul;14(7):634-46
Molecular cancer research : MCR 2016 Jul;14(7):634-46
ILEI drives epithelial to mesenchymal transition and metastatic progression in the lung cancer cell line A549.
Song Q, Sheng W, Zhang X, Jiao S, Li F
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Feb;35(2):1377-82
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Feb;35(2):1377-82
Establishment of a TGFβ-induced post-transcriptional EMT gene signature.
Hussey GS, Link LA, Brown AS, Howley BV, Chaudhury A, Howe PH
PloS one 2012;7(12):e52624
PloS one 2012;7(12):e52624
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCBP1 Antibody (1G2) [H00005093-M01] - Analysis of PCBP1 expression in transfected 293T cell line by PCBP1 monoclonal antibody (M01), clone 1G2.Lane 1: PCBP1 transfected lysate(37.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PCBP1 Antibody (1G2) [H00005093-M01] - Detection limit for recombinant GST tagged PCBP1 is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PCBP1 Antibody (1G2) [H00005093-M01] - Analysis of monoclonal antibody to PCBP1 on HeLa cell . Antibody concentration 10 ug/ml.
