H00005093-M01

antibody from Abnova Corporation

Targeting: PCBP1 hnRNP-E1, hnRNP-X, HNRPE1, HNRPX

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005093-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005093-M01, RRID:AB_894227

Product name

PCBP1 monoclonal antibody (M01), clone 1G2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PCBP1.

Antigen sequence

IPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLE
GPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAM
MHGGTGFAGIDSSSPEVKGYWASLDASTQT


Isotype

IgG

Antibody clone number

1G2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PCBP1 expression in transfected 293T cell line by PCBP1 monoclonal antibody (M01), clone 1G2.Lane 1: PCBP1 transfected lysate(37.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PCBP1 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PCBP1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol