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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP9827a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP9827a, RRID:AB_10614804
- Product name
- PDK2 Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Chronic CSE treatment induces the growth of normal oral keratinocytes via PDK2 upregulation, increased glycolysis and HIF1α stabilization.
Sun W, Chang SS, Fu Y, Liu Y, Califano JA
PloS one 2011 Jan 19;6(1):e16207
PloS one 2011 Jan 19;6(1):e16207
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "PDK2 Antibody (Cat.# AP9827a) western blot analysis in RD cell line and mouse heart,rat heart lysates (35ug/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow)."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- PDK2 Antibody (Cat. #AP9827a) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PDK2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- PDK2 Antibody (Cat. #AP9827a) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
