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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB7220 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7220, RRID:AB_1574320
- Product name
- DGAT2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of DGAT2.
- Antigen sequence
C-KLEHPTQQDIDLYH- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members.
Cases S, Stone SJ, Zhou P, Yen E, Tow B, Lardizabal KD, Voelker T, Farese RV Jr
The Journal of biological chemistry 2001 Oct 19;276(42):38870-6
The Journal of biological chemistry 2001 Oct 19;276(42):38870-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DGAT2 polyclonal antibody (Cat # PAB7220, 0.5 ug/mL) staining of human liver lysate in the third lane (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing DGAT2 (lane 1) and probed with DGAT2 polyclonal antibody (Cat # PAB7220) (mock transfection in lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DGAT2 polyclonal antibody (Cat # PAB7220, 3 ug/mL) staining of paraffin embedded human liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
