RQ7391

antibody from NSJ Bioreagents

Targeting: ME1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7391

Provider

NSJ Bioreagents

Product name

Malic enzyme 1 Antibody / ME1

Antibody type

Monoclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Mouse

Antibody clone number

5E5F7

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Malic enzyme 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human A549, 2) human HeLa, 3) rat testis, 4) rat brain, 5) mouse testis and 6) mouse brain tissue lysate with Malic enzyme 1 antibody. Predicted molecular weight ~64 kDa and ~55 kDa (two isoforms).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human spleen tissue with Malic enzyme 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with Malic enzyme 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat testis tissue with Malic enzyme 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.