OAAB02523

antibody from Aviva Systems Biology

Targeting: NR5A1 AD4BP, ELP, FTZ1, FTZF1, hSF-1, SF-1, SF1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB02523

Provider

Aviva Systems Biology

Product name

NR5A1 antibody Affinity Purified Rabbit Polyclonal antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of NR5A1 Antibody (N-term) (Cat#. AP5106a) in HepG2 cell lysates (35ug/lane).NR5A1 (arrow) was detected using the purified Pab.

Sample type

HepG2 cell line lysates

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

NR5A1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NR5A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Sample type

human spleen tissue

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

NR5A1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Sample type

HepG2 cells

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol